Field surveillance has revealed a high prevalence of PRV-3 in clinically healthy salmonids in Italy

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-10-03 DOI:10.1016/j.aqrep.2025.103131

Petra Drzewnioková , Romy Lucon Xiccato , Francesca Bruno , Claudia Casarotto , Matteo Mazzucato , Eleonora Franzago , Marica Toson , Niccolò Vendramin , Anna Toffan

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Abstract

Piscine orthoreovirus genotype 3 (PRV-3) has been reported in several European countries, and in Italy it was detected only once in 2017 without any associated disease outbreak. This study aimed to investigate the presence of PRV-3 in the area with the highest salmonids production in Italy. Samples were collected between September 2023 and May 2024 from three regions of northeastern Italy. Pooled samples were screened by a generic PRV real-time RT-PCR, and positive samples were subsequently characterized as PRV-3. The geographic distribution of PRV-3 was visualised and risk factors analysis was performed. Of the 267 tested specimens, 37.1 % yielded positive results, corresponding to 43.6 % positive farms in the investigated area. Phylogenetic analyses confirmed that the Italian PRV-3 strains clustered within group 3b. The highest positivity was observed in Salmo trutta (49.1 %), followed by Oncorhynchus mykiss (38.7 %) and Salmo marmoratus (24.3 %). No signs of diseases or history of mortality were observed from the positive batches of fish. Apparently healthy wild fish also tested positive. These results suggest that PRV-3 is widespread in both farmed and wild salmonid populations in the investigated area and that it does not cause specific health issues under the current Italian farming conditions and wild environment.

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现场监测显示，意大利临床健康鲑鱼中PRV-3的流行率很高

几个欧洲国家报告了鱼类正呼肠孤病毒基因型3 (PRV-3)，而意大利在2017年仅检测到一次，没有任何相关疾病暴发。本研究旨在调查PRV-3在意大利鲑鱼产量最高的地区的存在情况。样本是在2023年9月至2024年5月期间从意大利东北部的三个地区收集的。通过通用PRV实时RT-PCR筛选合并样本，阳性样本随后被鉴定为PRV-3。可视化PRV-3的地理分布并进行危险因素分析。在267个检测标本中，37.1% %产生阳性结果，与调查地区43.6% %的阳性农场相对应。系统发育分析证实意大利PRV-3毒株聚集在3b群中。阳性率最高的是小鲑（49.1 %），其次是小鲑（38.7 %）和小鲑（24.3 %）。从阳性批次的鱼中未观察到疾病迹象或死亡史。显然健康的野生鱼类也检测呈阳性。这些结果表明，PRV-3在调查地区的养殖和野生鲑鱼种群中广泛存在，并且在目前意大利的养殖条件和野生环境下，它不会造成特定的健康问题。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.