Pharmacological characterization of the neuropeptide F receptor (NPFR) in Macrobrachium rosenbergii

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-10-01 DOI:10.1016/j.aqrep.2025.103132

Yue-Miao Yu , Ke-Xin Yu , Li Sun , Dong-Ji Song , Da-Yan Hu , Shao-Kui Yi , Yang Li

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Abstract

The neuropeptide F receptor (NPFR) is a critical regulator of energy homeostasis in invertebrates, orchestrating feeding behavior, nutrient assimilation, and reproductive functions. We report the successful cloning and functional characterization of NPFR from the giant freshwater prawn Macrobrachium rosenbergii. Structural analysis confirmed that the cloned sequence is a typical GPCR with seven transmembrane domains and conserved motifs DRY and NPLLY. Sequence alignment showed high homology (>90 %) with other long-arm shrimp species, supporting evolutionary conservation within the Palaemonidae family. Quantitative real-time PCR (qRT-PCR) revealed ubiquitous expression of mrNPFR, with the highest levels in the intestinal tract, followed by hemolymph and eyestalk ganglia, suggesting multifunctional roles in energy homeostasis and neuroendocrine regulation. Functional assays using luciferase reporter systems demonstrated that NPF-II was the most potent cAMP inhibitor, while NPF-III exhibited the highest efficacy in activating the MAPK/ERK pathway. NPF-I showed the highest activation efficacy for the NFAT pathway. This study provides foundational data for understanding NPFR’s physiological functions in M. rosenbergii and offers insights for precision feeding strategies in aquaculture.

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罗氏沼虾神经肽F受体（NPFR）的药理特性

神经肽F受体（NPFR）是无脊椎动物能量稳态的关键调节因子，调控摄食行为、营养吸收和生殖功能。本文报道了罗氏沼虾NPFR基因的成功克隆和功能鉴定。结构分析证实克隆序列为典型的GPCR，具有7个跨膜结构域和保守基序DRY和NPLLY。序列比对显示与其他长臂虾种具有高度同源性（>90 %），支持长臂虾科的进化保守性。实时荧光定量PCR （Quantitative real-time PCR, qRT-PCR）结果显示，mrNPFR在肠道中表达水平最高，其次是血淋巴和眼柄神经节，提示其在能量稳态和神经内分泌调节中具有多种功能。使用荧光素酶报告系统进行的功能分析表明，NPF-II是最有效的cAMP抑制剂，而NPF-III在激活MAPK/ERK通路方面表现出最高的功效。npf - 1对NFAT通路的激活效果最高。该研究为了解罗氏沼虾NPFR的生理功能提供了基础数据，并为水产养殖的精准饲养策略提供了见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.