Effects of compound probiotics on the growth of Conger myriaster, water quality parameters, and microbial communities in industrial recirculating aquaculture systems

IF 3.7 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2025-10-03 DOI:10.1016/j.aqrep.2025.103138

Xinyu Zhao , Bao Shi , Chenbai Wang , Hanliang Cheng , Chenggang Wang , Xiaodong Ma , Kewen Yan , Yan Chen

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引用次数: 0

Abstract

The aim of this study was to determine the effects of adding compound probiotics to culture water on fish growth, water quality parameters, and microbial communities in industrial recirculating aquaculture systems (RAS) of Conger myriaster. Two systems were established: a control group system without any compound probiotics and a test group system with compound probiotics added to the culture water (1.0 × 10⁹ CFU/mL of viable bacteria, a dosage of 8 mL/m³, and a frequency of 1 time/7 d). The addition of compound probiotics to culture water in industrial RAS significantly improved the growth performance of C. myriaster (P < 0.05). Additionally, it significantly reduced the levels of ammonia nitrogen (NH₄⁺-N), nitrate nitrogen (NO₃⁻-N), nitrite nitrogen (NO₂⁻-N), total nitrogen (TN), and chemical oxygen demand (COD) in the culture tanks and biofilters in the test group on day 70 (P < 0.05). It also increased the average removal rates of NH₄⁺-N, NO₃⁻-N, NO₂⁻-N, TN^, COD, total phosphorus (TP), and PO₄^3--P in the culture tanks and biofilter water in the test group. The relative abundance of beneficial bacteria related to water purification was significantly increased in the culture water, biofilter water, and biofilter carriers in the test group at the end of the experiment (P < 0.05). Furthermore, the compound probiotics significantly reduced the influence of environmental factors on the microbial community in the water of the test group. Therefore, it played a dual role in regulating water quality and bacterial community structure. The bacterial communities in the water of the culture tanks and biofilters of the test group were mainly involved in functional roles such as chemoheterotrophy, aerobic chemoheterotrophy, and nitrite respiration. Overall, adding compound probiotics to culture water significantly improved the C. myriaster growth and water quality parameters, regulated the structure and function of the microbial community in the RAS, and promoted the degradation of nitrogenous compounds and organic matter.

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复合益生菌对工业循环水养殖系统中肉豆荚生长、水质参数和微生物群落的影响

本研究旨在研究在工业循环水养殖系统（RAS）中，在养殖水中添加复合益生菌对长肉豆豆鱼生长、水质参数和微生物群落的影响。建立两种体系：不添加复合益生菌的对照组体系和在培养水中添加复合益生菌的试验组体系（活菌量1.0 × 109 CFU/mL，投加量8 mL/m3，投加频率1次/7 d）。在工业RAS养殖水中添加复合益生菌可显著提高肉豆蔻菌的生长性能（P <； 0.05）。试验第70天，试验组培养池和生物滤池中氨氮（NH4+-N）、硝态氮（NO3−-N）、亚硝酸盐氮（NO2−-N）、总氮（TN）和化学需氧量（COD）水平显著降低（P <； 0.05）。提高了试验组培养池和生物滤池水中NH4+-N、NO3−-N、NO2−-N、TN、COD、总磷（TP）、PO43—P的平均去除率。实验结束时，试验组培养水、生物滤池水和生物滤池载体中与净水相关的有益菌相对丰度均显著增加（P <； 0.05）。此外，复合益生菌显著降低了环境因素对试验组水中微生物群落的影响。因此，它具有调节水质和细菌群落结构的双重作用。试验组培养池和生物滤池水中的细菌群落主要参与趋化异养、好氧趋化异养、亚硝酸盐呼吸等功能作用。综上所述，在培养水中添加复合益生菌显著改善了肉豆蔻菌的生长和水质参数，调节了RAS中微生物群落的结构和功能，促进了氮化合物和有机物的降解。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.