Praj K. Patel , Preisha Mishra , Habiba K. Ashour , Neil R. Mandar , Safa Mbarki , Yong Mao , Suneel Kumar , Francois Berthiaume , Aaron D. Mazzeo
{"title":"Antibacterial efficacy of combined atmospheric cold plasma and hydrogen peroxide treatment on a wound surrogate","authors":"Praj K. Patel , Preisha Mishra , Habiba K. Ashour , Neil R. Mandar , Safa Mbarki , Yong Mao , Suneel Kumar , Francois Berthiaume , Aaron D. Mazzeo","doi":"10.1016/j.bbrep.2025.102296","DOIUrl":null,"url":null,"abstract":"<div><div>This study aims to understand the potential combined effects of treating wound-like tissue surfaces with cold plasma (CP) and hydrogen peroxide. We assess how CP treatment generated by a surface dielectric barrier discharge (SDBD) device achieves bacterial inactivation on two test surfaces: agar plates, representing a surface with uniform topology, and muscle tissue from a thin-sliced chicken breast, representing a non-uniform topology mimicking a wound-like surface. A 10-min CP treatment inactivates <em>Escherichia coli (E. coli)</em> with up to 7 log reduction in colony-forming units (CFU) on a smooth agar surface; however, on chicken breast, the same treatment yields a 0.88 log reduction. By comparison, the common antiseptic H<sub>2</sub>O<sub>2</sub> (3 %) yields a 1.06 log CFU reduction on chicken breast after 10 min of treatment. Simultaneous treatment with CP and H<sub>2</sub>O<sub>2</sub> increases <em>E. coli</em> inactivation to 1.69 log CFU. Bacterial inactivation is less efficient on the chicken tissue than on smooth agar surfaces. Furthermore, the CP-H<sub>2</sub>O<sub>2</sub> combination significantly improves bacterial inactivation, which can be further enhanced by extending treatment time. This work demonstrates an approach to evaluating the efficacy of combining CP with liquid antimicrobial treatments on an accessible wound surrogate with complex morphology and biochemistry. This approach has the potential to serve as a fast method to screen candidate treatments before performing animal studies.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":"44 ","pages":"Article 102296"},"PeriodicalIF":2.2000,"publicationDate":"2025-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry and Biophysics Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405580825003838","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This study aims to understand the potential combined effects of treating wound-like tissue surfaces with cold plasma (CP) and hydrogen peroxide. We assess how CP treatment generated by a surface dielectric barrier discharge (SDBD) device achieves bacterial inactivation on two test surfaces: agar plates, representing a surface with uniform topology, and muscle tissue from a thin-sliced chicken breast, representing a non-uniform topology mimicking a wound-like surface. A 10-min CP treatment inactivates Escherichia coli (E. coli) with up to 7 log reduction in colony-forming units (CFU) on a smooth agar surface; however, on chicken breast, the same treatment yields a 0.88 log reduction. By comparison, the common antiseptic H2O2 (3 %) yields a 1.06 log CFU reduction on chicken breast after 10 min of treatment. Simultaneous treatment with CP and H2O2 increases E. coli inactivation to 1.69 log CFU. Bacterial inactivation is less efficient on the chicken tissue than on smooth agar surfaces. Furthermore, the CP-H2O2 combination significantly improves bacterial inactivation, which can be further enhanced by extending treatment time. This work demonstrates an approach to evaluating the efficacy of combining CP with liquid antimicrobial treatments on an accessible wound surrogate with complex morphology and biochemistry. This approach has the potential to serve as a fast method to screen candidate treatments before performing animal studies.
期刊介绍:
Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.