Antibacterial efficacy of combined atmospheric cold plasma and hydrogen peroxide treatment on a wound surrogate

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Praj K. Patel , Preisha Mishra , Habiba K. Ashour , Neil R. Mandar , Safa Mbarki , Yong Mao , Suneel Kumar , Francois Berthiaume , Aaron D. Mazzeo
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Abstract

This study aims to understand the potential combined effects of treating wound-like tissue surfaces with cold plasma (CP) and hydrogen peroxide. We assess how CP treatment generated by a surface dielectric barrier discharge (SDBD) device achieves bacterial inactivation on two test surfaces: agar plates, representing a surface with uniform topology, and muscle tissue from a thin-sliced chicken breast, representing a non-uniform topology mimicking a wound-like surface. A 10-min CP treatment inactivates Escherichia coli (E. coli) with up to 7 log reduction in colony-forming units (CFU) on a smooth agar surface; however, on chicken breast, the same treatment yields a 0.88 log reduction. By comparison, the common antiseptic H2O2 (3 %) yields a 1.06 log CFU reduction on chicken breast after 10 min of treatment. Simultaneous treatment with CP and H2O2 increases E. coli inactivation to 1.69 log CFU. Bacterial inactivation is less efficient on the chicken tissue than on smooth agar surfaces. Furthermore, the CP-H2O2 combination significantly improves bacterial inactivation, which can be further enhanced by extending treatment time. This work demonstrates an approach to evaluating the efficacy of combining CP with liquid antimicrobial treatments on an accessible wound surrogate with complex morphology and biochemistry. This approach has the potential to serve as a fast method to screen candidate treatments before performing animal studies.
常压冷等离子体联合双氧水治疗创面替代物的抗菌效果
本研究旨在了解用冷等离子体(CP)和过氧化氢处理伤口样组织表面的潜在联合效应。我们评估了由表面介质阻挡放电(SDBD)装置产生的CP处理如何在两个测试表面上实现细菌灭活:琼脂板,代表具有均匀拓扑的表面,以及来自薄切片鸡胸肉的肌肉组织,代表模拟伤口样表面的不均匀拓扑。10分钟的CP处理灭活大肠杆菌(E. coli),在光滑的琼脂表面上菌落形成单位(CFU)减少7倍;然而,在鸡胸肉上,同样的处理产生0.88对数减少。相比之下,普通防腐剂H2O2(3%)在处理10分钟后对鸡胸肉产生1.06 log CFU的减少。CP和H2O2同时处理使大肠杆菌失活率达到1.69 log CFU。细菌在鸡组织上的失活效果不如在光滑的琼脂表面上。此外,CP-H2O2组合显著改善了细菌的失活,延长处理时间可以进一步增强细菌的失活。这项工作展示了一种评估CP联合液体抗菌治疗对具有复杂形态和生物化学的可接近伤口替代物的疗效的方法。这种方法有可能在进行动物研究之前作为一种快速筛选候选治疗方法。
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来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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