Sulphide and oleic acid synergism in accelerating mcl-PHA biopolymer production in Pseudomonas aeruginosa MCC 5300 by modulating electron transport system

IF 2.2 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemistry and Biophysics Reports Pub Date : 2025-09-30 DOI:10.1016/j.bbrep.2025.102286

Raghavendra Paduvari, Divyashree Somashekara

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引用次数: 0

Abstract

Environmental concerns raised by petroleum-based plastics have sparked research on eco-friendly biodegradable polymers as alternatives. The medium chain length polyhydroxyalkanoates (mcl-PHA) are one such elastomeric polymer produced by a few bacteria that find various industrial, agricultural and biomedical applications. Besides its vast application, the low yield of wild-type bacterial strains and yield reduction due to reduced growth during prolonged cultivation in nutrient-limiting conditions limit industrial mcl-PHA production. In the present study, Pseudomonas aeruginosa MCC 5300 produced mcl-PHA of about 44.7 % CDW in tryptic soy broth (TSB) media containing oleic acid at 24 h of growth. The sulphide in media enhanced mcl-PHA content up to 86.5 % CDW in TSB media containing oleic acid at 24 h of growth. This is the first report of high mcl-PHA production at a short duration of 24 h in nutrient-enriched conditions. The oleic acid inhibited cytochrome c oxidase activity, shifting the electron flow from ubiquinol to cytochrome c pool. The sulphide increased the expression of bd-oxidase and enhanced electron flux through it, causing a rapid decline in the cellular NADH levels to maintain proton gradient and energy generation. The NADH reduction is compensated by excess mcl-PHA accumulated in bacteria. Hence, mcl-PHA maintains cellular redox-homeostasis during respiration using bd-oxidase.

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硫化物和油酸通过调节电子传递系统加速铜绿假单胞菌MCC 5300中mcl-PHA生物聚合物生产的协同作用

石油基塑料引发的环境问题引发了对生态友好的可生物降解聚合物替代品的研究。中链长聚羟基烷酸酯（mcl-PHA）是一种由几种细菌产生的弹性体聚合物，在工业、农业和生物医学领域有着广泛的应用。除了其广泛的应用之外，野生型菌株的低产量以及在营养限制条件下长时间培养导致的生长减少导致的产量降低限制了mcl-PHA的工业生产。在本研究中，铜绿假单胞菌MCC 5300在含有油酸的胰蛋白酶豆汤（TSB）培养基中生长24 h时产生的mcl-PHA CDW约为44.7%。在含油酸的TSB培养基中，培养基中的硫化物使mcl-PHA含量在生长24 h时达到86.5% CDW。这是首次报道在营养丰富的条件下，在短时间内24小时内高产出mcl-PHA。油酸抑制细胞色素c氧化酶活性，将电子从泛醇转移到细胞色素c池。硫化物增加了bd氧化酶的表达，增强了通过它的电子通量，导致细胞内NADH水平迅速下降，以维持质子梯度和能量产生。NADH的减少被细菌中积累的过量mcl-PHA所补偿。因此，mcl-PHA在呼吸过程中使用bd氧化酶维持细胞氧化还原稳态。

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来源期刊

Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

191

审稿时长

59 days

期刊介绍： Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.