Vegetative to generative1 (Vgt1) is an enhancer affecting flowering time and jasmonate signaling in maize by promoting the expression of Zea mays Related to APETALA 2.7

IF 6.9 1区生物学 Q1 PLANT SCIENCES

Plant Physiology Pub Date : 2025-10-03 DOI:10.1093/plphys/kiaf468

Johan Zicola, Blaise Weber, Xiaoyu Tu, Rechien Bader, Dimitrios Zisis, Stijn Aesaert, Silvio Salvi, Pawel Krajewski, Mieke Van Lijsebettens, Chuanshun Li, Yangmeihui Li, Silin Zhong, Stefan Scholten, Franziska Turck, Maike Stam

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引用次数: 0

Abstract

Transcriptional enhancers participate in cell and tissue differentiation in all multicellular organisms. Here, we characterized the candidate enhancer Vegetative to generative1 (Vgt1), a major QTL for flowering time in maize. Transgenic lines containing an inverted repeat that induces DNA methylation at Vgt1 showed early flowering and an accelerated growth rate during early development. DNA methylation of Vgt1 was associated with the downregulation of the AP2-like floral repressor ZmRap2.7 in specific leaf tissues at the early stages of maize development. In line with Vgt1 regulating ZmRap2.7, chromosome conformation capture data showed that Vgt1 physically interacts with the ZmRap2.7 transcription start site. Finally, chromatin immunoprecipitation of transiently expressed ZmRap2.7 in protoplasts indicated that this transcription factor binds to the promoters of several hundred genes. These genes include many genes that are differentially expressed in maize lines with and without extra DNA methylation at Vgt1. Altogether, we show that ZmRap2.7 is transcriptionally controlled by Vgt1 and is involved in regulating flowering time and other biological pathways, such as jasmonate signaling.

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Vgt1 （Vegetative to generative1）是一种通过促进与aptala相关的玉米基因表达而影响玉米开花时间和茉莉酸信号转导的促进剂

转录增强子参与所有多细胞生物的细胞和组织分化。在这里，我们对候选增强子Vgt1进行了表征，这是玉米开花时间的一个主要QTL。含有诱导Vgt1位点DNA甲基化的倒置重复序列的转基因品系在发育早期开花提前，生长速度加快。Vgt1的DNA甲基化与玉米发育早期特定叶片组织中ap2样花抑制因子ZmRap2.7的下调有关。染色体构象捕获数据显示，与Vgt1调控ZmRap2.7一致，Vgt1与ZmRap2.7转录起始位点存在物理相互作用。最后，原生质体中瞬时表达的ZmRap2.7的染色质免疫沉淀表明，该转录因子与数百个基因的启动子结合。这些基因包括许多在Vgt1有或没有额外DNA甲基化的玉米系中差异表达的基因。总之，我们发现ZmRap2.7受Vgt1的转录控制，并参与调节开花时间和其他生物学途径，如茉莉酸信号传导。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology 生物-植物科学

CiteScore

12.20

自引率

5.40%

发文量

535

审稿时长

2.3 months

期刊介绍： Plant Physiology® is a distinguished and highly respected journal with a rich history dating back to its establishment in 1926. It stands as a leading international publication in the field of plant biology, covering a comprehensive range of topics from the molecular and structural aspects of plant life to systems biology and ecophysiology. Recognized as the most highly cited journal in plant sciences, Plant Physiology® is a testament to its commitment to excellence and the dissemination of groundbreaking research. As the official publication of the American Society of Plant Biologists, Plant Physiology® upholds rigorous peer-review standards, ensuring that the scientific community receives the highest quality research. The journal releases 12 issues annually, providing a steady stream of new findings and insights to its readership.