PscCYP716A1-mediated brassinolide biosynthesis increases lead tolerance and enrichment in poplar

IF 5.7 2区 生物学 Q1 PLANT SCIENCES
Jie Yang , Keran Zhu , Xiaoxi Chen , Feifei Tian , Chengyu Han , Zhikun Liang , Kaixuan Xiao , Ruixue Hao , Meng Liu , Xiangyu Zhou , Xueqin Wan , Qinglin Liu , Fan Zhang
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引用次数: 0

Abstract

Urban soil lead (Pb) pollution poses a pressing threat to ecosystems and public health. Phytoremediation has emerged as an effective solution to address this issue. Poplars are widely employed in urban greening owing to their fast growth and high biomass, rendering them prime candidates for Pb remediation. However, their innate remediation capacity remains constrained, therefore, it needs to further enhance by genetic improvement. Transcriptome sequencing (RNA-seq) has identified that PscCYP716A1, a gene involved in brassinosteroid (BR) synthesis, is upregulated under Pb stress, indicating a potential role in Pb tolerance. This prompted a research question: Can PscCYP716A1 improve poplar's Pb tolerance? In this study, we obtained PscCYP716A1-overexpression poplars (OE lines, OE-13 and OE-42) and RNA interference PscCYP716A1-expression poplars (RNAi lines, RI-33 and RI-23). The overexpression poplars significantly enhanced Pb tolerance, and increased Pb accumulation compared to wild-type (WT). Further analysis revealed that PscCYP716A1 enhances Pb tolerance by improving the antioxidant system and Pb chelation. However, when exogenous brassinazole (BR biosynthesis inhibitor, BRZ) was sprayed on poplar under Pb stress, all advantages of OE lines disappeared. According to the results of analysis, the improvement of antioxidant and chelation capacities conferred by PscCYP716A1 may be related to its participation in the BR synthesis pathway. In summary, our results revealed the physiological mechanism and detoxification processes facilitated by PscCYP716A1, and underscored its potential as a genetic tool for enhancing phytoremediation in Pb-contaminated urban environments. These findings provide a theoretical foundation and technical support for the application of engineered poplars in remediating soil Pb pollution.
psccyp716a1介导的油菜素内酯生物合成增加了杨树对铅的耐受性和富集。
城市土壤铅污染对生态系统和公众健康构成了严重威胁。植物修复已成为解决这一问题的有效方法。杨树生长快,生物量高,在城市绿化中得到广泛应用,是铅修复的首选材料。然而,它们的先天修复能力仍然有限,因此需要通过遗传改良进一步增强。转录组测序(RNA-seq)发现,参与油菜素内酯(BR)合成的基因PscCYP716A1在铅胁迫下上调,表明其可能在铅耐受性中起作用。这引发了一个研究问题:PscCYP716A1能否提高杨树的铅耐受性?本研究获得了psccyp716a1过表达杨树(OE系、OE-13和OE-42)和RNA干扰表达psccyp716a1的杨树(RNAi系、RI-33和RI-23)。与野生型(WT)相比,过表达杨树对Pb的耐受性显著增强,Pb积累量显著增加。进一步分析发现PscCYP716A1通过改善抗氧化系统和Pb螯合作用增强Pb耐受性。然而,在铅胁迫下施用外源油菜素唑(BR生物合成抑制剂,BRZ)后,OE系的所有优势都消失了。根据分析结果,PscCYP716A1增强抗氧化和螯合能力可能与其参与BR合成途径有关。总之,我们的研究结果揭示了PscCYP716A1促进的生理机制和解毒过程,并强调了其作为加强铅污染城市环境中植物修复的遗传工具的潜力。研究结果为工程杨树修复土壤铅污染提供了理论基础和技术支持。
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来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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