Genome sequencing and comparative genome analysis of Clostridium perfringens of animal origin

IF 3.5 3区医学 Q3 IMMUNOLOGY

Microbial pathogenesis Pub Date : 2025-09-30 DOI:10.1016/j.micpath.2025.108079

Kumaragurubaran Karthik , Subbaiyan Anbazhagan , Prasad Thomas , Seeralan Manoharan

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Abstract

Clostridium perfringens, a ubiquitous organism that has zoonotic potential, is associated with enteric diseases in humans and various animals, largely due to its potent toxin production and increasing antimicrobial resistance (AMR). The present study involved whole genome sequencing and comparative genomic analysis of four C. perfringens isolates (from pig, dog, and cattle) collected in Tamil Nadu, India, along with 133 global strains of animal origin. The phylogenomic analysis revealed six major clusters and no correlation associated with geographic origin or host were observed. Multilocus sequence typing (MLST) defined sequence types (STs) of two Indian isolates (ST126 and ST200) whereas the remaining two isolates were novel., Pangenome analysis indicated an open genome structure, with 18.28 % core genes and 81.72 % accessory genes. Virulent gene profiling identified widespread presence of plc, pfoA, and cpe, especially among dog isolates, suggesting potential zoonotic risks. wgMLST and MST analysis showed clustering of toxinotype F isolates from dogs and higher genetic diversity for other toxintypess. AMR gene analysis revealed tetracycline resistance as the most prevalent, with several strains harbouring multidrug resistance genes. This study underscores the genetic diversity, adaptive evolution, and One Health significance of C. perfringens in animals and highlights the importance of genomics in understanding host-specific virulence and resistance patterns for effective disease surveillance and control.

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动物源产气荚膜梭菌基因组测序及比较基因组分析。

产气荚膜梭菌是一种普遍存在的具有人畜共患潜力的生物，与人类和各种动物的肠道疾病有关，主要是由于其强大的毒素产生和抗菌素耐药性（AMR）的增加。目前的研究包括在印度泰米尔纳德邦收集的4株产气荚膜荚膜菌（来自猪、狗和牛）的全基因组测序和比较基因组分析，以及133株来自动物的全球菌株。系统基因组分析显示6个主要聚类，与地理来源或宿主没有相关性。多位点序列分型（MLST）确定了两个印度分离株（ST126和ST200）的序列类型（STs），而其余两个分离株是新的。泛基因组分析显示，基因组结构开放，核心基因占18.28%，附属基因占81.72%。毒力基因分析发现，plc、pfoA和cpe广泛存在，特别是在犬分离株中，提示潜在的人畜共患风险。wgMLST和MST分析显示，来自犬的F型毒株具有聚类性，其他毒株具有较高的遗传多样性。AMR基因分析显示，四环素耐药最为普遍，一些菌株含有多药耐药基因。本研究强调了产气荚膜荚膜杆菌在动物中的遗传多样性、适应性进化和单一健康意义，并强调了基因组学在了解宿主特异性毒力和抗性模式方面的重要性，从而有效地监测和控制疾病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microbial pathogenesis 医学-免疫学

CiteScore

7.40

自引率

2.60%

发文量

472

审稿时长

56 days

期刊介绍： Microbial Pathogenesis publishes original contributions and reviews about the molecular and cellular mechanisms of infectious diseases. It covers microbiology, host-pathogen interaction and immunology related to infectious agents, including bacteria, fungi, viruses and protozoa. It also accepts papers in the field of clinical microbiology, with the exception of case reports. Research Areas Include: -Pathogenesis -Virulence factors -Host susceptibility or resistance -Immune mechanisms -Identification, cloning and sequencing of relevant genes -Genetic studies -Viruses, prokaryotic organisms and protozoa -Microbiota -Systems biology related to infectious diseases -Targets for vaccine design (pre-clinical studies)