Convergence of nanotechnology and CRISPR-based diagnostics.

IF 34.9 1区材料科学 Q1 MATERIALS SCIENCE, MULTIDISCIPLINARY

Nature nanotechnology Pub Date : 2025-10-02 DOI:10.1038/s41565-025-02018-8

Midori Johnston, Schan Dissanayake-Perera, James J Collins, Molly M Stevens, Can Dincer

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引用次数: 0

Abstract

In addition to its broad application in genome engineering and therapeutics, clustered regularly interspaced short palindromic repeats (CRISPR) technology provides field-deployable methods for the highly sensitive and selective detection of nucleic acids. From a diagnostic perspective, CRISPR-based assays hold clear clinical potential for identifying a range of both infectious and non-communicable diseases. In this Perspective we evaluate recent nanotechnologies and nanomaterials that have been engineered to interface with CRISPR systems on a nanoscale level to realize the full potential of this versatile diagnostic tool. We assess biomolecules such as enzymes and oligonucleotides, some of the more commonly used synthetic nanoparticles and detection platforms that integrate nanotechnologies in new and innovative ways. We discuss current trends and look ahead to future challenges and opportunities, including non-nucleic acid target detection, pre-amplification-free detection of nucleic acids, the development of wearable devices and integration with artificial intelligence workflows.

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纳米技术和基于crispr的诊断技术的融合。

除了在基因组工程和治疗学中的广泛应用外，聚集规律间隔短回文重复序列（CRISPR）技术还为高灵敏度和选择性的核酸检测提供了可现场部署的方法。从诊断的角度来看，基于crispr的分析在识别一系列传染性和非传染性疾病方面具有明确的临床潜力。在这个角度，我们评估了最近的纳米技术和纳米材料，这些纳米技术和纳米材料已经被设计成在纳米水平上与CRISPR系统接口，以实现这种多功能诊断工具的全部潜力。我们评估生物分子，如酶和寡核苷酸，一些更常用的合成纳米粒子和检测平台，以新的和创新的方式整合纳米技术。我们讨论了当前的趋势，展望了未来的挑战和机遇，包括非核酸目标检测、核酸的预扩增检测、可穿戴设备的发展以及与人工智能工作流程的集成。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature nanotechnology 工程技术-材料科学：综合

CiteScore

59.70

自引率

0.80%

发文量

196

审稿时长

4-8 weeks

期刊介绍： Nature Nanotechnology is a prestigious journal that publishes high-quality papers in various areas of nanoscience and nanotechnology. The journal focuses on the design, characterization, and production of structures, devices, and systems that manipulate and control materials at atomic, molecular, and macromolecular scales. It encompasses both bottom-up and top-down approaches, as well as their combinations. Furthermore, Nature Nanotechnology fosters the exchange of ideas among researchers from diverse disciplines such as chemistry, physics, material science, biomedical research, engineering, and more. It promotes collaboration at the forefront of this multidisciplinary field. The journal covers a wide range of topics, from fundamental research in physics, chemistry, and biology, including computational work and simulations, to the development of innovative devices and technologies for various industrial sectors such as information technology, medicine, manufacturing, high-performance materials, energy, and environmental technologies. It includes coverage of organic, inorganic, and hybrid materials.