Surface visualisation of bacterial biofilms using neutral atom microscopy.

IF 1.9 4区工程技术 Q3 MICROSCOPY

Journal of microscopy Pub Date : 2025-10-03 DOI:10.1111/jmi.70038

Nick A von Jeinsen, David J Ward, Matthew Bergin, Sam M Lambrick, David M Williamson, Richard M Langford, Lisa F Dawson, Vibhuti Rana, Sushma Shivaswamy, Xuening Zhou, Michelle Mikesh, Vernita D Gordon, Brendan W Wren, Katherine A Brown, Paul C Dastoor

引用次数: 0

Abstract

The scanning helium microscope (SHeM) is a new technology that uses a beam of neutral helium atoms to image surfaces non-destructively and with extreme surface sensitivity. Here, we present the application of the SHeM to image bacterial biofilms. We demonstrate that the SHeM uniquely and natively visualises the surface of the extracellular polymeric substance matrix in the absence of contrast agents and dyes and without inducing radiative damage.

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用中性原子显微镜观察细菌生物膜的表面。

扫描氦显微镜（SHeM）是一种利用中性氦原子束对表面进行非破坏性成像的新技术，具有极高的表面灵敏度。在这里，我们介绍了SHeM在细菌生物膜成像中的应用。我们证明，在没有造影剂和染料的情况下，SHeM独特而天然地显示细胞外聚合物基质的表面，并且不会引起辐射损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of microscopy 工程技术-显微镜技术

CiteScore

4.30

自引率

5.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit. The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens. Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.