Antifungal effects of metabolites from Arthrinium sp. 2-65 and identification of main active ingredients.

IF 4.2 2区生物学 Q2 MICROBIOLOGY

BMC Microbiology Pub Date : 2025-10-02 DOI:10.1186/s12866-025-04194-y

Huan Qu, Ershuai Yang, Mei Zuo, Zongze Li, Ludan Dai, Youping Su, Xiu Zhang, Yongbin Cheng, Yihan Chen, Yang Chen

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引用次数: 0

Abstract

Background: Using microbes and their metabolites as material to develop new biological fungicides is still vital for pesticide development. Our preliminary study found that the endophytic fungi Arthrinium sp. 2-65 of Thymus mongolicus (Ronniger) Ronniger showed a certain inhibitory effect on pathogenic fungi.

Results: In this study, the antifungal activity of Arthrinium sp. 2-65 was evaluated. The ethyl acetate extract of Arthrinium sp. 2-65 exhibited significant inhibitory activity against pathogenic fungi, especially Botrytis cinerea. The main compounds of Arthrinium sp. 2-65 metabolites were isolated and purified, and the two compounds were identified by infrared spectroscopy (IR), high-performance liquid chromatography (HPLC), ¹H nuclear magnetic resonance (NMR), ¹³C NMR, and high-resolution mass spectrometry (HRMS) as 2-hexyl-3-methylmaleic anhydride (A) and 2-carboxymethyl-3-n-hexylmaleic acid anhydride (B).

Conclusions: The main compounds (A and B) isolated and characterised from the fermentation broth of Arthrinium sp. 2-65 showed satisfactory inhibitory effects against pathogenic fungi, especially B. cinerea. These compounds could be used as potential molecules for the development of novel pesticides to control grey mould.

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Arthrinium sp. 2-65代谢物的抑菌作用及主要活性成分鉴定。

背景：以微生物及其代谢物为材料，开发新型生物杀菌剂对农药开发仍具有重要意义。初步研究发现，蒙古胸腺内生真菌Arthrinium sp. 2-65对病原菌有一定的抑制作用。结果：本研究对Arthrinium sp. 2-65的抑菌活性进行了评价。Arthrinium sp. 2-65乙酸乙酯提取物对病原菌有明显的抑制作用，对灰葡萄孢有明显的抑制作用。对Arthrinium sp. 2-65代谢产物的主要化合物进行了分离纯化，通过红外光谱（IR）、高效液相色谱（HPLC）、1H核磁共振（NMR）、13C核磁共振（NMR）和高分辨率质谱（HRMS）鉴定为2-己基-3-甲基马来酸酐(A)和2-羧甲基-3-正己基马来酸酐(B)。结论：从Arthrinium sp. 2-65发酵液中分离鉴定的主要化合物A和B对病原菌具有良好的抑菌作用，对灰霉的抑菌效果最好。这些化合物可作为开发防治灰霉病的新型农药的潜在分子。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

BMC Microbiology 生物-微生物学

CiteScore

7.20

自引率

0.00%

发文量

280

审稿时长

3 months

期刊介绍： BMC Microbiology is an open access, peer-reviewed journal that considers articles on analytical and functional studies of prokaryotic and eukaryotic microorganisms, viruses and small parasites, as well as host and therapeutic responses to them and their interaction with the environment.