Recombinant Proteins: A Molecular Tool to Understand Marine Adhesion and to Advance Biomaterials.

IF 9.6 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Alessandra Whaite, Emilie Duthoo, Mathilde Lefevre, Elise Hennebert, Patrick Flammang
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引用次数: 0

Abstract

Inspiration for innovation in healthcare regularly comes from observing the natural environment. Secreted adhesives are important for marine invertebrate attachment to submerged surfaces, and these systems have inspired investigations for better performing surgical adhesives. Natural marine adhesives are fundamentally proteins, therefore, most materials research has focused on the structure and function of proteinaceous components. Omics technologies have been used to identify proteins, but these candidates require further exploration to resolve function. Functional characterization begins by producing one specific protein in larger quantities with recombinant DNA technology. Recombinant proteins (RPs) are generally seen as mimics of individual marine adhesive proteins, representing a fundamental step in the development of bio-inspired glues. The literature details production of RPs from mussels, scallops, barnacles, tubeworms, ascidians, sea anemones, and sea stars, using bacteria, yeast, or insect and mammalian cells. Whole proteins, or components thereof, have been produced comprising the relevant amino acid sequences required for adhesion and have been investigated for use in healthcare via the production of materials that push the current limits of bio-inspired design. This is a thorough review of invertebrate marine adhesives investigated using biomimetic RPs, and a comprehensive overview of the innovative biomaterials designed utilizing knowledge from biological systems.

重组蛋白:了解海洋粘附和推进生物材料的分子工具。
医疗保健创新的灵感通常来自对自然环境的观察。分泌黏合剂对于海洋无脊椎动物附着在水下表面是很重要的,这些系统激发了对更好的外科黏合剂的研究。天然海洋粘合剂本质上是蛋白质,因此,大多数材料研究都集中在蛋白质成分的结构和功能上。组学技术已被用于鉴定蛋白质,但这些候选蛋白需要进一步探索以确定其功能。功能表征开始于用重组DNA技术大量生产一种特定蛋白质。重组蛋白(RPs)通常被视为单个海洋粘附蛋白的模拟物,代表了生物启发胶的发展的基本步骤。文献详细介绍了从贻贝、扇贝、藤壶、管虫、海鞘、海葵和海星中使用细菌、酵母或昆虫和哺乳动物细胞生产rp的方法。整个蛋白质,或其组成部分,已经生产出包含粘附所需的相关氨基酸序列,并且已经研究了通过生产推动当前生物启发设计极限的材料在医疗保健中的使用。本文全面回顾了利用仿生RPs研究的无脊椎动物海洋粘合剂,并全面概述了利用生物系统知识设计的创新生物材料。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advanced Healthcare Materials
Advanced Healthcare Materials 工程技术-生物材料
CiteScore
14.40
自引率
3.00%
发文量
600
审稿时长
1.8 months
期刊介绍: Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.
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