Enhanced Bacterial Degradation of Azo dyes by Escherichia sp. PP001: Optimization, Kinetics, Degradation Pathway, Phytotoxicity and Textile Wastewater Treatment

IF 3 4区环境科学与生态学 Q2 ENVIRONMENTAL SCIENCES

Water, Air, & Soil Pollution Pub Date : 2025-10-04 DOI:10.1007/s11270-025-08529-3

Mohanasundram Sudha, Arul Ponniah Saranya, Ganeshamoorthy Bakiyaraj, Mariappan Mariappan, Natesan Sivakumar

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Abstract

Azo dyes are organic, artificial colorants with a wide range of applications as textile coloring agents. The disposal of textile effluent negatively impacts soil and water ecosystems; hence, enhancing the microbial degradation of azo dyes is essential for the textile industry's disposal treatment. The prime focus of this research is studying the bacterial degradation of azo textile dyes, Direct Green 28 (DG-28) and Direct Blue 71 (DB-71). Taxonomic studies of the isolated strain revealed that it was Escherichia sp. PP001 (GenBank: KP770133.1), and it has the potential to degrade azo dyes. The culture conditions were optimized under various physiological parameters such as dye concentrations, pH, temperature, and agitation. The maximum decolorization of 99% was achieved for both dyes at pH 7, 37 °C, with agitation of 60 rpm (revolutions per minute). Biosorption kinetics fit pseudo-zero-order and diffusion models, indicating surface adsorption and intraparticle diffusion. GC–MS (Gas Chromatography Mass Spectroscopy) analysis elucidated metabolite degradation pathways. Physicochemical properties of the treated and untreated textile effluent were examined and compared. The results indicate a reduction of 97.63% in biological oxygen demand (BOD), 96.23% in chemical oxygen demand (COD), 64.56% in total dissolved solids (TDS), 76.80% in total solids (TS), and 85.50% in total suspended solids (TSS) in the treated textile effluent. The non-toxic nature of Escherichia sp. PP001-treated dyes and textile effluent were confirmed through phytotoxicity studies. Consequently, the treated effluent can be recommended for irrigation.

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细菌对偶氮染料的降解：优化、动力学、降解途径、植物毒性和纺织废水处理

偶氮染料是一种有机的人造着色剂，作为纺织品着色剂有着广泛的应用。纺织废水处理对土壤和水生态系统产生负面影响；因此，加强对偶氮染料的微生物降解对纺织工业的处置处理至关重要。本研究的主要重点是研究细菌降解偶氮纺织染料，直接绿28 （DG-28）和直接蓝71 （DB-71）。分离菌株的分类学研究表明，该菌株为Escherichia sp. PP001 (GenBank: KP770133.1)，具有降解偶氮染料的潜力。在染料浓度、pH、温度、搅拌等生理参数下对培养条件进行优化。两种染料在pH值为7、37℃、转速为60转/分钟的条件下，脱色率达到99%。生物吸附动力学符合伪零级和扩散模型，表明表面吸附和颗粒内扩散。GC-MS（气相色谱-质谱）分析阐明了代谢物的降解途径。对处理后的纺织废水和未处理的纺织废水进行了理化性能测试和比较。结果表明，处理后的纺织废水生物需氧量（BOD）降低97.63%，化学需氧量（COD）降低96.23%，总溶解固体（TDS）降低64.56%，总固体（TS）降低76.80%，总悬浮固体（TSS）降低85.50%。通过植物毒性研究，证实了pp001处理染料和纺织废水的无毒性。因此，处理后的废水可以推荐用于灌溉。图形抽象

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来源期刊

Water, Air, & Soil Pollution 环境科学-环境科学

CiteScore

4.50

自引率

6.90%

发文量

448

审稿时长

2.6 months

期刊介绍： Water, Air, & Soil Pollution is an international, interdisciplinary journal on all aspects of pollution and solutions to pollution in the biosphere. This includes chemical, physical and biological processes affecting flora, fauna, water, air and soil in relation to environmental pollution. Because of its scope, the subject areas are diverse and include all aspects of pollution sources, transport, deposition, accumulation, acid precipitation, atmospheric pollution, metals, aquatic pollution including marine pollution and ground water, waste water, pesticides, soil pollution, sewage, sediment pollution, forestry pollution, effects of pollutants on humans, vegetation, fish, aquatic species, micro-organisms, and animals, environmental and molecular toxicology applied to pollution research, biosensors, global and climate change, ecological implications of pollution and pollution models. Water, Air, & Soil Pollution also publishes manuscripts on novel methods used in the study of environmental pollutants, environmental toxicology, environmental biology, novel environmental engineering related to pollution, biodiversity as influenced by pollution, novel environmental biotechnology as applied to pollution (e.g. bioremediation), environmental modelling and biorestoration of polluted environments. Articles should not be submitted that are of local interest only and do not advance international knowledge in environmental pollution and solutions to pollution. Articles that simply replicate known knowledge or techniques while researching a local pollution problem will normally be rejected without review. Submitted articles must have up-to-date references, employ the correct experimental replication and statistical analysis, where needed and contain a significant contribution to new knowledge. The publishing and editorial team sincerely appreciate your cooperation. Water, Air, & Soil Pollution publishes research papers; review articles; mini-reviews; and book reviews.