Chromosome architecture affects virulence and competitiveness in Agrobacterium tumefaciens C58

IF 12.5 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Advances Pub Date : 2025-10-03 DOI:10.1126/sciadv.adx7408

Ephraim Aliu, Keunsub Lee, Ram Sanath-Kumar, Christopher Youngstrom, Lander Geadelmann, Dibyajyoti Pramanik, Qing Ji, Xindan Wang, Kan Wang

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Abstract

Chromosome architecture plays a crucial role in bacterial adaptation, yet its direct impact remains unclear. Different bacterial species and even strains within the same species exhibit diverse chromosomal configurations, including a single circular or linear chromosome, two circular chromosomes, or a circular-linear combination. To investigate how these architectures shape bacterial behavior, we generated near-isogenic strains representing each configuration in Agrobacterium tumefaciens C58, an important soil bacterium widely used for plant genetic transformation. Strains with a single-chromosome architecture, whether linear or circular, exhibited faster growth, enhanced stress tolerance, and greater interstrain competitiveness. In contrast, bipartite chromosome strains showed higher virulence gene expression and enhanced transient plant transformation efficiency, suggesting a pathogenic adaptation. Whole-transcriptome analysis revealed architecture-dependent gene expression patterns, underscoring the profound impact of chromosome organization on Agrobacterium fitness and virulence. These findings highlight how chromosome structure influences bacterial adaptation and shapes evolutionary trajectories.

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染色体结构影响农杆菌C58的毒力和竞争能力

染色体结构在细菌适应中起着至关重要的作用，但其直接影响尚不清楚。不同的细菌物种甚至同一物种内的菌株表现出不同的染色体构型，包括单个圆形或线性染色体，两个圆形染色体或圆形-线性组合。为了研究这些结构如何影响细菌的行为，我们在农杆菌C58中生成了代表每种结构的近等基因菌株，农杆菌C58是一种广泛用于植物遗传转化的重要土壤细菌。具有单染色体结构的菌株，无论是线性还是圆形，都表现出更快的生长，更强的抗逆性和更强的菌株间竞争能力。相比之下，双染色体菌株表现出更高的毒力基因表达和更高的瞬时植物转化效率，表明其具有致病适应性。全转录组分析揭示了结构依赖的基因表达模式，强调了染色体组织对农杆菌适应性和毒力的深刻影响。这些发现强调了染色体结构如何影响细菌适应和塑造进化轨迹。

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来源期刊

Science Advances 综合性期刊-综合性期刊

CiteScore

21.40

自引率

1.50%

发文量

1937

审稿时长

29 weeks

期刊介绍： Science Advances, an open-access journal by AAAS, publishes impactful research in diverse scientific areas. It aims for fair, fast, and expert peer review, providing freely accessible research to readers. Led by distinguished scientists, the journal supports AAAS's mission by extending Science magazine's capacity to identify and promote significant advances. Evolving digital publishing technologies play a crucial role in advancing AAAS's global mission for science communication and benefitting humankind.