Light-Powered Transport of Organic Anions by Microbial Rhodopsins.

IF 4.6 2区化学 Q2 CHEMISTRY, PHYSICAL

The Journal of Physical Chemistry Letters Pub Date : 2025-10-02 DOI:10.1021/acs.jpclett.5c02551

Simiao Shen,Shoichiro Akita,Joji Wada,Mako Eguchi,Takashi Tsukamoto,Kwang-Hwan Jung,Yuki Sudo,Takashi Kikukawa

{"title":"Light-Powered Transport of Organic Anions by Microbial Rhodopsins.","authors":"Simiao Shen,Shoichiro Akita,Joji Wada,Mako Eguchi,Takashi Tsukamoto,Kwang-Hwan Jung,Yuki Sudo,Takashi Kikukawa","doi":"10.1021/acs.jpclett.5c02551","DOIUrl":null,"url":null,"abstract":"Microbial rhodopsins are photoactive membrane proteins known for transporting small inorganic ions such as H+, Cl-, and Na+. Their compact structure─comprising seven transmembrane helices─has long been thought to limit their substrate range to such ions. Here, we report that several anion-pumping rhodopsins can also transport organic anions. In particular, a rhodopsin from cyanobacteria transports bulky organic anions, including those with a benzene ring, with volumes up to ∼120 Å3─five times larger than Cl-. These anions bind in the dark state and are translocated upon photoactivation, via a mechanism similar to Cl-. Notably, only anions with pKa values below 2 are transported, suggesting that negative charge is essential for binding. This study provides the first evidence that naturally occurring proteins can use light to transport organic compounds across membranes. These findings broaden the functional scope of microbial rhodopsins and open new possibilities for light-driven transport of organic ions.","PeriodicalId":62,"journal":{"name":"The Journal of Physical Chemistry Letters","volume":"98 1","pages":"10528-10535"},"PeriodicalIF":4.6000,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Physical Chemistry Letters","FirstCategoryId":"1","ListUrlMain":"https://doi.org/10.1021/acs.jpclett.5c02551","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, PHYSICAL","Score":null,"Total":0}

引用次数: 0

Abstract

Microbial rhodopsins are photoactive membrane proteins known for transporting small inorganic ions such as H+, Cl-, and Na+. Their compact structure─comprising seven transmembrane helices─has long been thought to limit their substrate range to such ions. Here, we report that several anion-pumping rhodopsins can also transport organic anions. In particular, a rhodopsin from cyanobacteria transports bulky organic anions, including those with a benzene ring, with volumes up to ∼120 Å3─five times larger than Cl-. These anions bind in the dark state and are translocated upon photoactivation, via a mechanism similar to Cl-. Notably, only anions with pKa values below 2 are transported, suggesting that negative charge is essential for binding. This study provides the first evidence that naturally occurring proteins can use light to transport organic compounds across membranes. These findings broaden the functional scope of microbial rhodopsins and open new possibilities for light-driven transport of organic ions.

查看原文本刊更多论文

微生物紫红质对有机阴离子的光传输。

微生物紫红质是一种具有光活性的膜蛋白，以运输小的无机离子如H+， Cl-和Na+而闻名。它们紧凑的结构──包括七个跨膜螺旋──长期以来一直被认为限制了它们的底物范围。在这里，我们报道了几种阴离子泵视紫红质也可以运输有机阴离子。特别是，蓝藻中的视紫红质可以输送体积高达~ 120 Å3的有机阴离子，其体积是Cl-的5倍。这些阴离子在暗态结合，并通过类似于Cl-的机制在光活化下易位。值得注意的是，只有pKa值低于2的阴离子被传输，这表明负电荷是结合所必需的。这项研究提供了第一个证据，证明天然存在的蛋白质可以利用光跨膜运输有机化合物。这些发现拓宽了微生物视紫红质的功能范围，为有机离子的光驱动运输开辟了新的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

The Journal of Physical Chemistry Letters CHEMISTRY, PHYSICAL-NANOSCIENCE & NANOTECHNOLOGY

CiteScore

9.60

自引率

7.00%

发文量

1519

审稿时长

1.6 months

期刊介绍： The Journal of Physical Chemistry (JPC) Letters is devoted to reporting new and original experimental and theoretical basic research of interest to physical chemists, biophysical chemists, chemical physicists, physicists, material scientists, and engineers. An important criterion for acceptance is that the paper reports a significant scientific advance and/or physical insight such that rapid publication is essential. Two issues of JPC Letters are published each month.