Molecular survey of the haemosporidians Haemoproteus and Leucocytozoon in Culicoides (Diptera: Ceratopogonidae) from the Brazilian Amazon.

IF 1.2 4区农林科学 Q2 Veterinary

Revista Brasileira De Parasitologia Veterinaria Pub Date : 2025-09-29 eCollection Date: 2025-01-01 DOI:10.1590/S1984-29612025048

Daniel Antônio Braga Lee, Israel de Souza Pinto, Paulo Vitor Cadina Arantes, Maria Clara Alves Santarém, Maria Luiza Felippe-Bauer, João Vitor Dos Santos Alves da Silva, Rosangela Zacarias Machado, Marcos Rogério André

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引用次数: 0

Abstract

Haemosporidians belonging to the subgenus Haemoproteus (Parahaemoproteus) and the genus Leucocytozoon rely on dipteran vectors for transmission, with biting midges (Culicoides spp.) and black flies (Simuliidae), respectively, playing essential roles in their life cycles. However, little is known about the potential role of Culicoides species as vectors for haemosporidians outside Europe and Asia. This study aimed to investigate the presence of Haemoproteus spp. and Leucocytozoon spp. DNA in Culicoides spp. from the Brazilian Amazon. A total of 345 midges (95 Culicoides foxi, 218 Culicoides hylas, and 32 Culicoides leopoldoi) were collected between February 2022 and February 2023 in the Amazon National Park, Pará. Specimens were morphologically identified, and DNA was extracted using the TRIzol technique. PCR targeting the endogenous cox-1 gene confirmed successful DNA extraction in 86.7% (299/345) of samples. Nested PCR assays targeting the cytB gene of Haemoproteus spp. and Leucocytozoon spp. did not detect DNA in any sample. The absence of detection suggests that the sampled Culicoides species are unlikely vectors, possibly due to feeding preferences or ecological limitations. Future studies targeting both DNA and sporozoites in salivary glands of engorged female Culicoides spp. are needed to clarify the vectors involved in the transmission of haemosporidians.

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巴西亚马逊地区库蠓血孢子虫、血变形虫和白细胞虫的分子调查。

血孢子虫属血变形亚属（副血变形亚属）和白细胞虫属依靠双翅目媒介传播，其中吸血蠓和黑蝇分别在其生命周期中起重要作用。然而，对于库蠓在欧洲和亚洲以外作为血吸虫病媒介的潜在作用知之甚少。本研究旨在调查巴西亚马孙地区库蠓属（Culicoides）中是否存在嗜血杆菌属（Haemoproteus）和白细胞原虫属（Leucocytozoon）的DNA。2022年2月至2023年2月在帕尔亚马逊国家公园共采集蠓345只，其中福库蚊95只，黄库蚊218只，利波库蚊32只。对标本进行形态学鉴定，并采用TRIzol技术提取DNA。针对内源性cox-1基因的PCR结果显示，86.7%（299/345）的样品DNA提取成功。针对嗜血杆菌和白细胞原虫cytB基因的巢式PCR检测未检测到任何样本中的DNA。没有检测到库蠓，这表明采样的库蠓不太可能是媒介，可能是由于摄食偏好或生态限制。未来的研究需要同时针对库蠓雌虫的DNA和唾液腺孢子体，以明确参与血孢子虫传播的媒介。

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来源期刊

Revista Brasileira De Parasitologia Veterinaria PARASITOLOGY-VETERINARY SCIENCES

CiteScore

2.60

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： La revista es un órgano de difusión del Colegio Brasileño de Parasitología Veterinaria, con una especificidad dentro de esa área, la difusión de los resultados de la investigación brasileña en las áreas de Helmintología, Protozoología, Entomología y agentes transmitidos por artrópodos, relacionados con la salud animal.