Decoding Rhizosphere Synergies: Pseudomonas and Bacillus Enhance Microbiome-Mediated Suppression of Rhizoctonia solani in Sugar Beet.

IF 3.1 2区 农林科学 Q2 PLANT SCIENCES
Kexin Li, Tai Li, Yonglong Liu, Bingchen Zou, Gui Geng, Yao Xu, Jiahui Liu, Yuguang Wang
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Abstract

Sugar beet is a crucial sugar crop with substantial economic and nutritional value. The occurrence of damping-off disease severely impacts sugar beet quality and yield. Here, we successfully isolated two endophytes from sugar beet, and it follow as Bacillus albus SB-3 and Pseudomonas chlororaphis SB-35, via morphological observation and molecular identification. Both SB-3 and SB-35 exhibited nitrogen-fixing and potassium mobilization capabilities, with SB-35 demonstrating additional traits including phosphate solubilization, potassium mobilization. SB-3 and SB-35 promoted the growth of sugar beet, resulting in increased biomass, and improved soil available nutrient. Besides, SB-3 and SB-35 had also extracellular protease activities and inhibited the mycelium growth of Rhizoctonia solani. In independent pot experiments, SB-3 and SB-35 exhibited significantly controlling the damping-off of seedlings for sugar beet. Further analysis indicated that SB-3 and SB-35 may alter microbial community structure, reducing the abundance of Rhizoctonia solani, promoting the recruitment of beneficial microorganisms, such as Hypocrea, Peziza, and Talaromyces, to occupy ecological niches, thereby reducing the numbers of pathogen. The two bacterial strains modulated the diversity and community structure of rhizosphere microorganisms, suggesting a microbiome-mediated mechanism underlying their host-beneficial effects. This study advances our understanding of harnessing endophytes to enhance sugar beet productivity and suppressing sugar beet damping-off caused by Rhizoctonia solani.

解码根际协同作用:假单胞菌和芽孢杆菌增强微生物介导的甜菜根核孢子菌抑制。
甜菜是重要的糖料作物,具有重要的经济和营养价值。消湿病的发生严重影响甜菜品质和产量。本研究通过形态学观察和分子鉴定,成功从甜菜中分离到2种内生菌,分别为白芽孢杆菌SB-3和绿绿假单胞菌SB-35。SB-3和SB-35都表现出固氮和钾动员能力,SB-35还表现出磷酸盐增溶和钾动员等其他特性。SB-3和SB-35促进了甜菜的生长,增加了生物量,改善了土壤速效养分。此外,SB-3和SB-35还具有胞外蛋白酶活性,并能抑制茄枯丝核菌菌丝的生长。在独立盆栽试验中,SB-3和SB-35对甜菜幼苗减湿有显著的控制作用。进一步分析表明,SB-3和SB-35可能改变了微生物群落结构,降低了茄枯丝核菌的丰度,促进了有益微生物如Hypocrea、Peziza和Talaromyces的聚集,占据生态位,从而减少了病原体的数量。这两种菌株调节了根际微生物的多样性和群落结构,表明它们对宿主有益的机制是由微生物组介导的。本研究促进了我们对利用内生菌提高甜菜产量和抑制甜菜枯枯病的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Phytopathology
Phytopathology 生物-植物科学
CiteScore
5.90
自引率
9.40%
发文量
505
审稿时长
4-8 weeks
期刊介绍: Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.
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