Tonoplast sugar transporter StTST1 mediates vacuolar sugar partitioning and abiotic stress tolerance in potato

IF 5.7 2区 生物学 Q1 PLANT SCIENCES
Jin Wang , Md Abu Kawocha , Tengfei Liu , Tiantian Liu , Jingjing Guo , Shulan Hu , Yi Liu , Shengxuan Liu , Lin Chen , Bihua Nie , Botao Song
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Abstract

Sugar transporters play pivotal roles in plant growth, development, and stress responses. However, the function of sugar transporters in potato (Solanum tuberosum) is still obscure. In this study, the function of potato tonoplast sugar transporter 1 (StTST1) in subcellular sugar compartmentation and abiotic stress tolerance was characterized. Heterologous expression assays in Saccharomyces cerevisiae (strain W303) demonstrated that StTST1 mediates sucrose transporting into the vacuole. Moreover overexpression and RNA interference (RNAi) of StTST1 in potato altered leafy sugar content including sucrose, glucose, and fructose without affecting the activity of key metabolic enzymes. Intriguingly, RNAi-mediated suppression of StTST1 enhanced freezing tolerance and compromised drought tolerance. In contrast, overexpression of StTST1 enhanced drought tolerance but reduced freezing tolerance. Our results demonstrate that StTST1 dynamically regulates subcellular sugar partitioning and differentially modulates freezing and drought stress responses. These findings highlight the potential of targeted manipulation of sugar transporters to modulate crop resilience to multiple abiotic stresses.
tono质体糖转运蛋白StTST1介导马铃薯液泡糖分配和非生物胁迫抗性。
糖转运蛋白在植物生长发育和逆境反应中起着关键作用。然而,糖转运蛋白在马铃薯(Solanum tuberosum)中的作用尚不清楚。本研究研究了马铃薯叶绿体糖转运蛋白1 (StTST1)在亚细胞糖区隔和非生物胁迫耐受中的功能。在酿酒酵母菌(菌株W303)中的异源表达实验表明,StTST1介导蔗糖转运到液泡中。此外,StTST1在马铃薯中的过表达和RNA干扰(RNAi)改变了包括蔗糖、葡萄糖和果糖在内的叶糖含量,但不影响关键代谢酶的活性。有趣的是,rnai介导的StTST1抑制增强了抗冻性,降低了抗旱性。相比之下,StTST1的过表达增强了抗旱性,但降低了抗冻性。我们的研究结果表明,StTST1动态调节亚细胞糖分配,并差异调节冰冻和干旱胁迫反应。这些发现强调了有针对性地操纵糖转运蛋白以调节作物对多种非生物胁迫的抗逆性的潜力。
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来源期刊
Plant Physiology and Biochemistry
Plant Physiology and Biochemistry 生物-植物科学
CiteScore
11.10
自引率
3.10%
发文量
410
审稿时长
33 days
期刊介绍: Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.
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