Video rate widefield sensitive polarized light microscopy by spectrally structured illumination.

IF 3.3 2区物理与天体物理 Q2 OPTICS

Optics letters Pub Date : 2025-10-01 DOI:10.1364/OL.570077

Hugo Laviec, Sylvain Rivet, Matthieu Dubreuil, Bruno Boulbry, Mikhail Indenbom, Yann Le Grand

引用次数: 0

Abstract

In this Letter, we propose a setup to realize quantitative and sensitive widefield microscopy of optical retardation and azimuth of a birefringent specimen at video rate. The originality of the technique lies in the spectral structuration of the microscope illumination using spectral coding of polarization. The optical retardation and azimuth map of the specimen are obtained from a combination of three intensity images captured after successive illumination of the microscope by three phase-shifted spectrally structured spectra from light-emitting diodes (LEDs). The high commutation speed of the LEDs and the passive nature of all optical elements allow the acquisition at video rate and potentially more.

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采用光谱结构照明的视频率宽视场敏感偏振光显微镜。

在这篇文章中，我们提出了一个装置，以实现定量和敏感的宽视场显微镜的光学延迟和方位角的双折射标本在视频速率。该技术的独创性在于利用偏振光谱编码实现显微镜照明的光谱结构。光学延迟和样品的方位图是从三个强度图像的组合后捕获的连续照明后的显微镜由三个相移的光谱结构光谱从发光二极管（led）。led的高换相速度和所有光学元件的无源特性允许以视频速率和潜在的更高速率进行采集。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Optics letters 物理-光学

CiteScore

6.60

自引率

8.30%

发文量

2275

审稿时长

1.7 months

期刊介绍： The Optical Society (OSA) publishes high-quality, peer-reviewed articles in its portfolio of journals, which serve the full breadth of the optics and photonics community. Optics Letters offers rapid dissemination of new results in all areas of optics with short, original, peer-reviewed communications. Optics Letters covers the latest research in optical science, including optical measurements, optical components and devices, atmospheric optics, biomedical optics, Fourier optics, integrated optics, optical processing, optoelectronics, lasers, nonlinear optics, optical storage and holography, optical coherence, polarization, quantum electronics, ultrafast optical phenomena, photonic crystals, and fiber optics. Criteria used in determining acceptability of contributions include newsworthiness to a substantial part of the optics community and the effect of rapid publication on the research of others. This journal, published twice each month, is where readers look for the latest discoveries in optics.