Mild increase of temperature from the thermoneutral zone inhibits reproductive activation in Syrian hamsters through epigenetic inhibition.

IF 4.1 4区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Lalsiamkima Hnamler, Amit Kumar Trivedi
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引用次数: 0

Abstract

The rapid increase in urbanization is drastically altering the habitat composition of the wild population. Urbanization is predominantly changing the landscape, composition of flora and fauna, availability of night light, and the rise in temperature. In the natural habitat, photoperiod and temperature are inseparable. In the present study, we examined the effect of mild temperature change from the thermoneutral zone of Syrian hamsters on reproduction-linked activities. To investigate the neuroendocrine mechanisms underlying heat stress effects on reproduction in hamsters, two experiments were performed on adult male animals. In experiment one, animals were divided into two groups (n = 5/group) and exposed to a long photoperiod (15L:9D) with either low (LT; 20 ± 2°C) or high temperature (HT; 32 ± 2°C). After 21 days, all animals were sampled. In experiment two, hamsters (n = 20) were divided equally into two groups and were exposed to the first short photoperiod of 8L:16D, but with low temperature (LT; 20 ± 2°C) or high temperature (HT; 32 ± 2°C). After 30 days, all animals were exposed to a long day (15L:9D), but animals from each temperature treatment were divided equally into two groups (n = 5/group). Half of the animals (n = 5) of low temperature remained in low temperature (LL group) while the remaining animals were moved to high temperature (LH group). Similarly, half of the animals (n = 5) of high temperature remained in high temperature (HH group), and the rest of the animals were moved to low temperature (HL group). Body mass and testicular volume were measured at different intervals. After 30 days of long-day treatment, the animals were sampled. Findings suggest that exposure to 3 weeks of high temperature attenuates testicular growth, coupled with low testosterone levels and downregulation of Kiss1, Eya3, Tshβ, GnRh, Tet1, Tet2, and Hat1, while upregulation of Dio3, GnIh, Dnmt1, Dnmt3A, Hdac1, and Hdac5 occurs in HT groups. Results from experiment two suggest that low temperature promotes, while high temperature attenuates reproduction and the linked phenomenon. Together, these findings suggest that high temperature modulates the reproductive responses of Syrian hamsters.

从热中性区轻微升高的温度通过表观遗传抑制抑制叙利亚仓鼠的生殖激活。
城市化的快速发展正在极大地改变野生种群的栖息地组成。城市化主要改变了景观、动植物的组成、夜间照明的可用性和温度的上升。在自然栖息地,光周期和温度是不可分割的。在本研究中,我们研究了来自叙利亚仓鼠热中性区的温和温度变化对生殖相关活动的影响。为了探讨热应激对仓鼠生殖影响的神经内分泌机制,我们对成年雄性仓鼠进行了两项实验。实验一,将动物分为两组(n = 5/组),分别在低温(20±2°C)和高温(32±2°C)下长时间(15L:9D)光照。21 d后,对所有动物取样。实验二,将20只仓鼠平均分为两组,分别在低温(LT; 20±2°C)和高温(HT; 32±2°C)条件下接受8L:16D的短光周期光照。30天后,所有动物都暴露在一个长白天(15L:9D),但每个温度处理的动物平均分为两组(n = 5/组)。半数(n = 5)低温动物留在低温组(LL组),其余动物移至高温组(LH组)。同样,一半的高温动物(n = 5)留在高温(HH组),其余动物移至低温(HL组)。在不同的时间间隔测量体重和睾丸体积。经过30天的长时间治疗,这些动物被取样。结果表明,暴露于3周的高温下,睾丸生长减慢,睾酮水平降低,Kiss1、Eya3、Tshβ、GnRh、Tet1、Tet2和Hat1下调,而在高温组,Dio3、GnIh、Dnmt1、Dnmt3A、Hdac1和Hdac5上调。实验二的结果表明,低温促进繁殖和相关现象,而高温则减弱繁殖和相关现象。总之,这些发现表明高温调节了叙利亚仓鼠的生殖反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Neuroendocrinology
Journal of Neuroendocrinology 医学-内分泌学与代谢
CiteScore
6.40
自引率
6.20%
发文量
137
审稿时长
4-8 weeks
期刊介绍: Journal of Neuroendocrinology provides the principal international focus for the newest ideas in classical neuroendocrinology and its expanding interface with the regulation of behavioural, cognitive, developmental, degenerative and metabolic processes. Through the rapid publication of original manuscripts and provocative review articles, it provides essential reading for basic scientists and clinicians researching in this rapidly expanding field. In determining content, the primary considerations are excellence, relevance and novelty. While Journal of Neuroendocrinology reflects the broad scientific and clinical interests of the BSN membership, the editorial team, led by Professor Julian Mercer, ensures that the journal’s ethos, authorship, content and purpose are those expected of a leading international publication.
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