Design and performance of a real-time RT-PCR assay for detection of influenza C viruses

IF 3.7 3区医学 Q2 VIROLOGY

Journal of Clinical Virology Pub Date : 2025-12-01 Epub Date: 2025-09-25 DOI:10.1016/j.jcv.2025.105874

Bo Shu , William G. Davis , Ji Liu , Beth K. Thielen , Sarah Bistodeau , Brian Lynch , Christine M. Warnes , Jimma Liddell , Anna K. Strain , Jaime Christensen , Phili Wong , Natasha Burnett , Todd C. Davis , Marie K. Kirby

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引用次数: 0

Abstract

Influenza C virus (ICV) usually causes a mild upper respiratory tract infection in children and those infected are frequently co-infected with other respiratory viruses. However, there have only been a few hundred documented cases of ICV infection in humans as of the end of 2024. To better understand the epidemiology and clinical impact of ICVs, we developed an influenza C real-time RT-PCR (InfC rRT-PCR) assay that targets a highly conserved region of the matrix gene segment of ICVs. The analytical sensitivity evaluation demonstrated that the InfC rRT-PCR assay was highly sensitive, as it was able to detect as few as five RNA copies per PCR reaction and had robust reactivity over a range of viral RNAs from historical and recent ICVs. The analytical specificity evaluation confirmed the assay did not cross-react with any influenza A or B viruses tested, including several animal-origin viruses, or other common non-influenza respiratory viruses. The performance evaluation on clinical specimens demonstrated the assay was highly sensitive and specific for the detection of ICVs.

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C型流感病毒实时RT-PCR检测方法的设计与性能

丙型流感病毒（ICV）通常在儿童中引起轻度上呼吸道感染，受感染者经常与其他呼吸道病毒合并感染。然而，截至2024年底，只有几百例记录在案的人类感染ICV病例。为了更好地了解icv的流行病学和临床影响，我们开发了一种针对icv基质基因片段高度保守区域的流感C实时RT-PCR （InfC rRT-PCR）检测。分析敏感性评估表明，InfC rRT-PCR检测是高度敏感的，因为它能够在每次PCR反应中检测到5个RNA拷贝，并且对来自历史和近期icv的一系列病毒RNA具有强大的反应性。分析特异性评价证实，该检测方法与测试的任何甲型或乙型流感病毒（包括几种动物源性病毒或其他常见的非流感呼吸道病毒）均无交叉反应。对临床标本的性能评价表明，该方法对ICVs的检测具有高度的敏感性和特异性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Virology 医学-病毒学

CiteScore

22.70

自引率

1.10%

发文量

149

审稿时长

24 days

期刊介绍： The Journal of Clinical Virology, an esteemed international publication, serves as the official journal for both the Pan American Society for Clinical Virology and The European Society for Clinical Virology. Dedicated to advancing the understanding of human virology in clinical settings, the Journal of Clinical Virology focuses on disseminating research papers and reviews pertaining to the clinical aspects of virology. Its scope encompasses articles discussing diagnostic methodologies and virus-induced clinical conditions, with an emphasis on practicality and relevance to clinical practice. The journal publishes on topics that include: • new diagnostic technologies • nucleic acid amplification and serologic testing • targeted and metagenomic next-generation sequencing • emerging pandemic viral threats • respiratory viruses • transplant viruses • chronic viral infections • cancer-associated viruses • gastrointestinal viruses • central nervous system viruses • one health (excludes animal health)