Dual-target electrochemical aptasensor based on Mil101(Fe)-CQD-TiO2 ternary composite for Cryptosporidium and cadmium ion detection

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-10-02 DOI:10.1007/s00604-025-07567-2

Indiphile Nompetsheni, Nithyadharsen Palaniyandy, Ntuthuko Wonderboy Hlongwa, Xolile Fuku

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Abstract

A Mil101 (Fe)-carbon quantum dot-titanium dioxide (Mil101(Fe)-CQD-TiO₂) ternary composite was prepared using the hydrothermal method. The composite was thoroughly characterized using various physicochemical techniques to investigate the crystallinity of the materials, the functional groups present, chemical vibrations, elemental composition, and morphology, confirming the formation of the ternary composite. The electrochemical properties of the ternary composite were then evaluated through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). This ternary composite served as an electrode modifier for aptasensor fabrication. The aptasensor exhibited an electroactive surface area of 3.22 cm², indicating a higher number of active sites for analyte interaction. The aptasensing platform was optimized and evaluated in phosphate buffer solution and real water samples using square wave voltammetry (SWV). Additionally, the sensor demonstrated a limit of detection (LOD) of 0.001 µM for Cryptosporidium and 0.073 µM for cadmium ions with high sensitivities of 9 × 10⁻⁴ mA/µM and 0.127 mA/µM in buffer solutions. The aptasensor demonstrated excellent selectivity, with recoveries ranging from 67 to 98.1% for Cryptosporidium and 72 to 89% for Cd²⁺.

Graphical Abstract

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基于Mil101(Fe)-CQD-TiO2三元复合材料的双靶电化学感应传感器用于隐孢子虫和镉离子检测。

采用水热法制备了Mil101(Fe)-碳量子点-二氧化钛（Mil101(Fe)-CQD-TiO2）三元复合材料。利用各种物理化学技术来研究材料的结晶度、存在的官能团、化学振动、元素组成和形态，对复合材料进行了彻底的表征，证实了三元复合材料的形成。然后通过循环伏安法（CV）和电化学阻抗谱（EIS）对三元复合材料的电化学性能进行了评价。该三元复合材料可作为电极修饰剂用于感应传感器的制备。配体传感器的电活性表面积为3.22 cm2，表明分析物相互作用的活性位点数量较多。利用方波伏安法（SWV）对适体感应平台进行了优化，并在磷酸盐缓冲溶液和实际水样中进行了评价。此外，该传感器对隐孢子虫的检测限（LOD）为0.001µM，对镉离子的检测限为0.073µM，在缓冲溶液中灵敏度分别为9 × 10-4 mA/µM和0.127 mA/µM。该传感器对隐孢子虫和Cd2+的检测回收率分别为67 ~ 98.1%和72 ~ 89%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.