HY5 integrates light and electrical signaling to trigger a jasmonate burst for nematode defense in tomato.

IF 15.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Nature Communications Pub Date : 2025-10-01 DOI:10.1038/s41467-025-63779-3

Ting Sun,Chaoyi Hu,Changan Zhu,Jiaxing Cai,Guoting Wang,Xiaojian Xia,Kai Shi,Yanhong Zhou,Jingquan Yu,Christine H Foyer,Jie Zhou

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Abstract

Plants have evolved sophisticated defense systems to protect against herbivory, including the systemic induction of jasmonic acid (JA) synthesis. However, the molecular mechanisms underlying this process remain poorly understood. Here, we report that root-knot nematode (RKN) attack induced a phyB-dependent accumulation of ELONGATED HYPOCOTYL 5 (HY5) in the leaves, which activates the expression of JA biosynthesis genes and HY5 itself in tomato. In addition, the systemic transmission of GLUTAMATE RECEPTOR-LIKE 3.5 (GLR3.5)-dependent electrical signals induced by RKN triggers the physical interaction between CALMODULIN 2 (CaM2) and HY5 to amplify the transcriptional regulation of HY5 and JA synthesis. HY5 functions as a systemic signal that moves from leaves to roots to maintain the electrical signaling from roots to leaves by activating GLR3.5 expression. Together, these results reveal a HY5-dependent systemic signaling cascade that integrates light and electrical signals to activate JA-mediated defense against nematodes in tomato.

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HY5整合光和电信号，触发茉莉酸爆发，以防御番茄线虫。

植物已经进化出复杂的防御系统来保护自己免受食草动物的侵害，包括系统诱导茉莉酸（JA）的合成。然而，这一过程背后的分子机制仍然知之甚少。本研究报道了根结线虫（root-knot nematode， RKN）侵毒诱导番茄叶片中伸长下胚轴5 （ELONGATED HYPOCOTYL 5, HY5）依赖phyb积累，激活JA生物合成基因和HY5自身的表达。此外，RKN诱导的谷氨酸受体样3.5 （GLR3.5）依赖性电信号的系统性传递触发了CALMODULIN 2 （CaM2）和HY5之间的物理相互作用，从而放大了HY5和JA合成的转录调控。HY5作为一个从叶片到根的系统性信号，通过激活GLR3.5的表达，维持从根到叶的电信号。总之，这些结果揭示了hy5依赖性的系统信号级联，该级联整合光和电信号来激活ja介导的番茄对线虫的防御。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature Communications Biological Science Disciplines-

CiteScore

24.90

自引率

2.40%

发文量

6928

审稿时长

3.7 months

期刊介绍： Nature Communications, an open-access journal, publishes high-quality research spanning all areas of the natural sciences. Papers featured in the journal showcase significant advances relevant to specialists in each respective field. With a 2-year impact factor of 16.6 (2022) and a median time of 8 days from submission to the first editorial decision, Nature Communications is committed to rapid dissemination of research findings. As a multidisciplinary journal, it welcomes contributions from biological, health, physical, chemical, Earth, social, mathematical, applied, and engineering sciences, aiming to highlight important breakthroughs within each domain.