{"title":"Phase-Sensitive Surface Plasmon Resonance Imaging with Polarization Modulation and Stokes Vector Measurement.","authors":"Baiqi Cui,Xiaoyin Liu,Jinbiao Ma,Jingyu Wu,Yunxiao Wang,Haiying Ding,Di Wang,Qingjun Liu,Fenni Zhang","doi":"10.1021/acssensors.5c02048","DOIUrl":null,"url":null,"abstract":"Phase-sensitive surface plasmon resonance (P-SPR) techniques offer superior sensitivity compared to conventional intensity-based SPR methods. However, current implementations, such as interference-based and polarization-based phase measurements, often face trade-offs between optical complexity and phase resolution, and typically lack access to full polarization information. Here, we report a compact and versatile Stokes vector-based polarization SPR imaging (Sp-SPRi) system that addresses these limitations through full Stokes vector analysis of imaging light after polarization modulation. This analysis enables the simultaneous acquisition of phase and multiple polarization parameters for enhanced molecular measurement. The Sp-SPRi system achieves a high phase sensitivity of 1.80 × 10-7 refractive index units (RIU), supporting kinetic, label-free detection and quantification of biomolecular interactions. We demonstrate its performance through kinetic measurements of protein binding, small molecule interactions, and in situ glycoprotein measurement of single cells. With its simplified optical configuration, microfluidic integration, and high-throughput imaging capabilities, Sp-SPRi provides a powerful platform for multidimensional biosensing. This work broadens the analytical scope of P-SPR, offering a robust and accessible approach for applications in point-of-care diagnostics, early disease detection, and drug screening.","PeriodicalId":24,"journal":{"name":"ACS Sensors","volume":"1 1","pages":""},"PeriodicalIF":9.1000,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Sensors","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acssensors.5c02048","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Phase-sensitive surface plasmon resonance (P-SPR) techniques offer superior sensitivity compared to conventional intensity-based SPR methods. However, current implementations, such as interference-based and polarization-based phase measurements, often face trade-offs between optical complexity and phase resolution, and typically lack access to full polarization information. Here, we report a compact and versatile Stokes vector-based polarization SPR imaging (Sp-SPRi) system that addresses these limitations through full Stokes vector analysis of imaging light after polarization modulation. This analysis enables the simultaneous acquisition of phase and multiple polarization parameters for enhanced molecular measurement. The Sp-SPRi system achieves a high phase sensitivity of 1.80 × 10-7 refractive index units (RIU), supporting kinetic, label-free detection and quantification of biomolecular interactions. We demonstrate its performance through kinetic measurements of protein binding, small molecule interactions, and in situ glycoprotein measurement of single cells. With its simplified optical configuration, microfluidic integration, and high-throughput imaging capabilities, Sp-SPRi provides a powerful platform for multidimensional biosensing. This work broadens the analytical scope of P-SPR, offering a robust and accessible approach for applications in point-of-care diagnostics, early disease detection, and drug screening.
期刊介绍:
ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.