DNA Nanostructures for Modular Growth Factor Delivery and Peripheral Nerve Repair.

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-10-02 DOI:10.1021/acs.nanolett.5c03151

Youngjin Choi,Su Jeong Park,Bo Kyung Cho,Jongmin Lee,Yejin Sung,Yang C Zeng,Nakwon Choi,William M Shih,Soo Hyun Kim,Dong-Hwee Kim,Seung-Woo Cho,Youngmee Jung,Ju Hee Ryu

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引用次数: 0

Abstract

Severe peripheral nerve injuries cause significant functional impairments due to limited regenerative capacity. Growth factors such as brain-derived neurotrophic factor (BDNF) and nerve growth factor promote neuronal differentiation and regeneration, but their controlled and efficient delivery remains challenging. Here, we present square block DNA nanostructures (SQBs) as a modular platform for the spatially controlled presentation of growth-factor-mimicking peptides. SQBs displaying 38 BDNF-mimicking peptides at 5 nm intervals enhanced the neuronal differentiation of human mesenchymal stem cells. Dual-ligand presentation was validated using fluorescein isothiocyanate and cyanine5.5, demonstrating ratio-controlled conjugation and colocalized delivery within single cells. In a sciatic nerve injury model, BDNF-functionalized SQBs modestly improved functional recovery, reduced muscle atrophy, and enhanced remyelination compared to the untreated crush group. Histological analysis revealed increased myelin sheath thickness and improved axonal integrity. These findings underscore potential SQBs as programmable and spatially precise delivery systems for neuroregenerative therapies and broader tissue repair strategies.

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模块化生长因子递送和周围神经修复的DNA纳米结构。

由于再生能力有限，严重的周围神经损伤会导致严重的功能损伤。脑源性神经营养因子（BDNF）和神经生长因子等生长因子可促进神经元的分化和再生，但其调控和有效递送仍具有挑战性。在这里，我们提出了方形块DNA纳米结构（sqb）作为一个模块化平台，用于空间控制生长因子模拟肽的呈现。在间隔5 nm处显示38个bdnf模拟肽的sqb可促进人间充质干细胞的神经元分化。使用异硫氰酸荧光素和花青素5.5验证了双配体的呈现，证明了比例控制的偶联和单细胞内的共定位传递。在坐骨神经损伤模型中，与未治疗的挤压组相比，bdnf功能化的SQBs适度改善了功能恢复，减少了肌肉萎缩，并增强了髓鞘再生。组织学分析显示髓鞘厚度增加，轴突完整性改善。这些发现强调了sqb作为神经再生治疗和更广泛的组织修复策略的可编程和空间精确递送系统的潜力。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.