Jihong Jiang, Yi Wu, Xiaoyu Shang, Meiru Jiang, Liping Zhu, Dongmei Hong, Enduo Feng, Guanghui An
{"title":"Surface-Enhanced Raman Spectroscopy for Propofol Detection in Postoperative Patient Whole Blood Samples","authors":"Jihong Jiang, Yi Wu, Xiaoyu Shang, Meiru Jiang, Liping Zhu, Dongmei Hong, Enduo Feng, Guanghui An","doi":"10.1016/j.snb.2025.138877","DOIUrl":null,"url":null,"abstract":"Propofol remains the first sedative choice due to its rapid onset and short distribution half-life. However, the narrow therapeutic window and pronounced inter-individual pharmacokinetic variability pose challenges in achieving appropriate sedation levels in diverse patients, calling for rapid and accurate quantification of propofol for its clinical safety. In this work, a sensitive and rapid surface-enhanced Raman scattering (SERS) platform based on vertically aligned Au nanorods array with enhancement factor >10<sup>7</sup> was fabricated for directly propofol detection in unprocessed postoperative blood samples. Achieving by rapid and selective derivatization of propofol with 2,6-dichloroquinone-4-chloroimide (DCQ) in alkaline conditions, the SERS platform presented excellent selectivity resulted by a strong 1278<!-- --> <!-- -->cm<sup>-1</sup> peak of indophenol. This assay displayed a linear response from 1<!-- --> <!-- -->ng<!-- --> <!-- -->mL<sup>-1</sup> to 20<!-- --> <!-- -->µg<!-- --> <!-- -->mL<sup>-1</sup> with a limit of detection of 0.38<!-- --> <!-- -->ng<!-- --> <!-- -->mL<sup>-1</sup> in 2<!-- --> <!-- -->min for each sample. Both the artificial simulated serum and clinical postoperative blood samples testing demonstrated excellent quantification performance of as-prepared SERS platform. Significantly, Comparative analysis with high-performance liquid chromatography (HPLC) showed a Deming slope of 0.95, confirming analytical interchangeability. Moreover, A subsequent cohort of 32 patients demonstrated a robust inverse correlation between SERS-derived propofol levels and Richmond Agitation-Sedation Scale scores (Spearman ρ=-0.80, p<0.0001), a single threshold of 1.17<!-- --> <!-- -->µg<!-- --> <!-- -->mL<sup>-1</sup> predicted moderate sedation to deep sedation with 100% sensitivity and 100% specificity. This work furnishes the first clinically validated, reagent less-cleanup SERS assay for intravenous anesthetic monitoring, offering a generic blueprint for real-time therapeutic-drug management in critical care.","PeriodicalId":425,"journal":{"name":"Sensors and Actuators B: Chemical","volume":"115 1","pages":""},"PeriodicalIF":3.7000,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors and Actuators B: Chemical","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1016/j.snb.2025.138877","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Propofol remains the first sedative choice due to its rapid onset and short distribution half-life. However, the narrow therapeutic window and pronounced inter-individual pharmacokinetic variability pose challenges in achieving appropriate sedation levels in diverse patients, calling for rapid and accurate quantification of propofol for its clinical safety. In this work, a sensitive and rapid surface-enhanced Raman scattering (SERS) platform based on vertically aligned Au nanorods array with enhancement factor >107 was fabricated for directly propofol detection in unprocessed postoperative blood samples. Achieving by rapid and selective derivatization of propofol with 2,6-dichloroquinone-4-chloroimide (DCQ) in alkaline conditions, the SERS platform presented excellent selectivity resulted by a strong 1278 cm-1 peak of indophenol. This assay displayed a linear response from 1 ng mL-1 to 20 µg mL-1 with a limit of detection of 0.38 ng mL-1 in 2 min for each sample. Both the artificial simulated serum and clinical postoperative blood samples testing demonstrated excellent quantification performance of as-prepared SERS platform. Significantly, Comparative analysis with high-performance liquid chromatography (HPLC) showed a Deming slope of 0.95, confirming analytical interchangeability. Moreover, A subsequent cohort of 32 patients demonstrated a robust inverse correlation between SERS-derived propofol levels and Richmond Agitation-Sedation Scale scores (Spearman ρ=-0.80, p<0.0001), a single threshold of 1.17 µg mL-1 predicted moderate sedation to deep sedation with 100% sensitivity and 100% specificity. This work furnishes the first clinically validated, reagent less-cleanup SERS assay for intravenous anesthetic monitoring, offering a generic blueprint for real-time therapeutic-drug management in critical care.
期刊介绍:
Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.