A-327 Rapid Antifungal Susceptibility Testing System for Yeasts Based on Fast Cell Growth

IF 6.3 2区医学 Q1 MEDICAL LABORATORY TECHNOLOGY

Clinical chemistry Pub Date : 2025-10-02 DOI:10.1093/clinchem/hvaf086.315

Zeqi Zhou, Xiuzhen Wu, Yaonan Lin, Ying Wang, Yuan Zhang, He Wang, Yan Su, Shigui Liu

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Abstract

Background Due to the growing burden of fungal infections and a recent rise in antifungal resistance, antifungal susceptibility testing (AFST) is of increasing importance. The common methods of AFST for yeasts have turnaround times of 24 to 48 h. Given the urgency of adequate antifungal treatment in invasive mycoses, rapid antifungal susceptibility testing is urgently needed in clinics to treat invasive fungal infections with the appropriate antifungal drugs and to slow the emergence of antifungal resistance. Methods Here, we demonstrate a rapid AFST by measuring the metabolism in fungal cells using resazurin and a medium that promotes the growth and metabolism of fungal cells (Fig). A total of 32 clinical fungal strains were randomly analyzed from the Peking Union Medical College Hospital. Species of these strains were commonly found in patients with systemic infections, including 9 Candida albicans, 5 Candida krusei, 3 Candida tropicalis, 3 Candida parapsilosis, 3 Candida lusitaniae, 7 Candida glabrata, and 2 Candida guilliermondii. The Sensititre YeastOne panel was performed according to the manufacturer*s instructions as reference methods. For the rapid AFST method, MICs were determined at 6 h of incubation. MICs for the YeastOne panel were read at 24 h of incubation. The overall agreement within +/- 2 dilutions by both methods was calculated against ten antifungal agents. Results Considering the Sensititre YeastOne Colorimetric Broth method as a comparator method, ten antifungal agents (?uconazole, itraconazole, voriconazole, posaconazole, isavuconazole, caspofungin, micafungin, anidulafungin, amphotericin B, and 5-flucytosine) showed an essential agreement of > 90%. Conclusion Therefore, this rapid AFST can be considered as an optional method that can obtain results and interpretations faster than previous methods. This method overcomes the limitation of slow growth in conventional methods and has the potential for the rapid diagnosis of candidemia and other clinical fungal infections.

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基于快速细胞生长的A-327型酵母药敏快速检测系统

随着真菌感染负担的增加和抗真菌耐药性的增加，抗真菌药敏试验（AFST）变得越来越重要。酵母AFST的常用方法周转时间为24至48小时。鉴于侵袭性真菌病需要充分的抗真菌治疗的紧迫性，临床迫切需要快速的抗真菌药敏试验，以使用适当的抗真菌药物治疗侵袭性真菌感染，并减缓抗真菌耐药性的出现。在这里，我们通过使用resazurin和促进真菌细胞生长和代谢的培养基来测量真菌细胞的代谢，从而证明了快速的AFST（图）。对北京协和医院32株临床真菌进行随机分析。这些菌株常见于全身感染患者，包括白色念珠菌9株、克氏念珠菌5株、热带念珠菌3株、副假丝酵母菌3株、卢西假丝酵母菌3株、光滑念珠菌7株、吉列蒙假丝酵母菌2株。根据制造商的说明书作为参考方法进行Sensititre YeastOne检测。对于快速AFST法，在孵育6 h时测定mic。在孵育24 h时读取YeastOne组的mic。两种方法在+/- 2稀释范围内的总体一致性是针对10种抗真菌剂进行计算的。结果以灵敏酵母素比色肉汤法为对照法，10种抗真菌药物(？乌康唑、伊曲康唑、伏立康唑、泊沙康唑、异唑康唑、卡泊芬津、米卡芬津、阿尼杜拉芬津、两性霉素B和5-氟胞嘧啶基本一致。90%。结论该方法可作为一种可选方法，比以往方法更快获得结果和解释。该方法克服了传统方法生长缓慢的局限性，具有快速诊断念珠菌病和其他临床真菌感染的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical chemistry 医学-医学实验技术

CiteScore

11.30

自引率

4.30%

发文量

212

审稿时长

1.7 months

期刊介绍： Clinical Chemistry is a peer-reviewed scientific journal that is the premier publication for the science and practice of clinical laboratory medicine. It was established in 1955 and is associated with the Association for Diagnostics & Laboratory Medicine (ADLM). The journal focuses on laboratory diagnosis and management of patients, and has expanded to include other clinical laboratory disciplines such as genomics, hematology, microbiology, and toxicology. It also publishes articles relevant to clinical specialties including cardiology, endocrinology, gastroenterology, genetics, immunology, infectious diseases, maternal-fetal medicine, neurology, nutrition, oncology, and pediatrics. In addition to original research, editorials, and reviews, Clinical Chemistry features recurring sections such as clinical case studies, perspectives, podcasts, and Q&A articles. It has the highest impact factor among journals of clinical chemistry, laboratory medicine, pathology, analytical chemistry, transfusion medicine, and clinical microbiology. The journal is indexed in databases such as MEDLINE and Web of Science.