A-264 Urine Culture Trends from 2022 to 2025: A Review of Microorganism Prevalence

IF 6.3 2区医学 Q1 MEDICAL LABORATORY TECHNOLOGY

Clinical chemistry Pub Date : 2025-10-02 DOI:10.1093/clinchem/hvaf086.253

Marina Bezerra, Rachel Petrola, Edlâny Milanez

{"title":"A-264 Urine Culture Trends from 2022 to 2025: A Review of Microorganism Prevalence","authors":"Marina Bezerra, Rachel Petrola, Edlâny Milanez","doi":"10.1093/clinchem/hvaf086.253","DOIUrl":null,"url":null,"abstract":"Background Urinary tract infections (UTIs) are among the leading causes of bacterial infections, affecting millions of people annually and impacting public health. These infections can affect any part of the urinary system, ranging from mild cases to complications like pyelonephritis and sepsis. Several factors can influence the development and severity of UTIs, including age, sex, and comorbidities such as diabetes mellitus. This study aims to analyze the epidemiological profile of UTIs in outpatients from a Brazilian population, investigating the distribution of etiological agents and their association with age, sex, diabetes, and bacterial colony counts. The use of big data for laboratory analysis provides a broad approach to understanding the behavior of these infections, their impact on clinical management, and corroboration with the literature. Methods This epidemiological, cross-sectional study analyzed the distribution of etiological agents in urine cultures from outpatients at a private laboratory in the Northeast region of Brazil. Data between January 2022 and February 2025 were extracted from the LIS system. Urinalysis and urine culture results were analyzed using Sysmex® and Vitek - 2 Compat ® equipment, considering bacterial colony counts and self-reported diabetes status. The population was stratified by sex and age. Results The population was mainly female (79%), with a mean age of 55 ± 22 years. Bacterial growth was observed in 22,326 (19.8%) of the urine cultures (N=112,506). The main pathogens identified were Escherichia coli (12%; N=13,259), Klebsiella pneumoniae (3.2%; N=3,652), Proteus mirabilis (0.9%; N=1,035), and Enterococcus faecalis (0.9%; N=1,002). Extended-spectrum beta-lactamase (ESBL)-positive cases accounted for 14% (N=1,450). Among the ESBL-positive cases, five patients had bacterial growth exceeding 1,000,000 CFU/mL (Klebsiella pneumoniae N=4, Proteus mirabilis N=1), with two cases of dual colonization (Klebsiella/E. coli and Proteus/E. coli). Among the patients, 15% (N=16,473) self-reported as diabetic. This group had a higher prevalence of ESBL-positive infections (18%, N=384), and in cases with more than one isolated microorganism, ESBL positivity was observed in 38%. Candida glabrata was more prevalent in diabetic patients. Conclusion The analysis revealed that most cases occurred in women, particularly those aged 18 to 50 years, with Escherichia coli as the most frequent pathogen. Diabetic patients had a higher prevalence of infections caused by ESBL-positive microorganisms, especially in mixed infections. Additionally, Candida glabrata was more prevalent in this group. The findings show that the prevalence of main UTI pathogens remains similar to that reported in recent years. This study emphasizes the importance of regularly updating epidemiological data to improve clinical management strategies for these infections.","PeriodicalId":10690,"journal":{"name":"Clinical chemistry","volume":"62 1","pages":""},"PeriodicalIF":6.3000,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Clinical chemistry","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1093/clinchem/hvaf086.253","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Background Urinary tract infections (UTIs) are among the leading causes of bacterial infections, affecting millions of people annually and impacting public health. These infections can affect any part of the urinary system, ranging from mild cases to complications like pyelonephritis and sepsis. Several factors can influence the development and severity of UTIs, including age, sex, and comorbidities such as diabetes mellitus. This study aims to analyze the epidemiological profile of UTIs in outpatients from a Brazilian population, investigating the distribution of etiological agents and their association with age, sex, diabetes, and bacterial colony counts. The use of big data for laboratory analysis provides a broad approach to understanding the behavior of these infections, their impact on clinical management, and corroboration with the literature. Methods This epidemiological, cross-sectional study analyzed the distribution of etiological agents in urine cultures from outpatients at a private laboratory in the Northeast region of Brazil. Data between January 2022 and February 2025 were extracted from the LIS system. Urinalysis and urine culture results were analyzed using Sysmex® and Vitek - 2 Compat ® equipment, considering bacterial colony counts and self-reported diabetes status. The population was stratified by sex and age. Results The population was mainly female (79%), with a mean age of 55 ± 22 years. Bacterial growth was observed in 22,326 (19.8%) of the urine cultures (N=112,506). The main pathogens identified were Escherichia coli (12%; N=13,259), Klebsiella pneumoniae (3.2%; N=3,652), Proteus mirabilis (0.9%; N=1,035), and Enterococcus faecalis (0.9%; N=1,002). Extended-spectrum beta-lactamase (ESBL)-positive cases accounted for 14% (N=1,450). Among the ESBL-positive cases, five patients had bacterial growth exceeding 1,000,000 CFU/mL (Klebsiella pneumoniae N=4, Proteus mirabilis N=1), with two cases of dual colonization (Klebsiella/E. coli and Proteus/E. coli). Among the patients, 15% (N=16,473) self-reported as diabetic. This group had a higher prevalence of ESBL-positive infections (18%, N=384), and in cases with more than one isolated microorganism, ESBL positivity was observed in 38%. Candida glabrata was more prevalent in diabetic patients. Conclusion The analysis revealed that most cases occurred in women, particularly those aged 18 to 50 years, with Escherichia coli as the most frequent pathogen. Diabetic patients had a higher prevalence of infections caused by ESBL-positive microorganisms, especially in mixed infections. Additionally, Candida glabrata was more prevalent in this group. The findings show that the prevalence of main UTI pathogens remains similar to that reported in recent years. This study emphasizes the importance of regularly updating epidemiological data to improve clinical management strategies for these infections.

查看原文本刊更多论文

A-264尿液培养趋势从2022年到2025年：微生物流行的回顾

尿路感染（uti）是细菌感染的主要原因之一，每年影响数百万人并影响公共卫生。这些感染可以影响泌尿系统的任何部分，从轻微的病例到肾盂肾炎和败血症等并发症。有几个因素可以影响尿路感染的发展和严重程度，包括年龄、性别和合并症，如糖尿病。本研究旨在分析巴西门诊患者尿路感染的流行病学特征，调查病因分布及其与年龄、性别、糖尿病和细菌菌落计数的关系。使用大数据进行实验室分析为了解这些感染的行为、它们对临床管理的影响以及与文献的佐证提供了广泛的方法。方法本流行病学横断面研究分析了巴西东北部地区一家私人实验室门诊患者尿液培养物中病原学因子的分布。从2022年1月至2025年2月的数据提取自LIS系统。使用Sysmex®和Vitek - 2 Compat®设备分析尿液分析和尿液培养结果，考虑细菌菌落计数和自我报告的糖尿病状况。人口按性别和年龄分层。结果患者以女性为主（79%），平均年龄55±22岁。22,326例（19.8%）尿液培养物（N=112,506）中有细菌生长。主要病原菌为大肠杆菌（12%，N=13,259）、肺炎克雷伯菌（3.2%，N=3,652）、奇异变形杆菌（0.9%，N=1,035）、粪肠球菌（0.9%，N=1,002）。广谱β -内酰胺酶（ESBL）阳性病例占14% （N=1,450）。在esbl阳性病例中，5例患者细菌生长超过1,000,000 CFU/mL（肺炎克雷伯菌N=4，奇异变形杆菌N=1）， 2例双重定植(克雷伯菌/E。大肠杆菌和变形杆菌/E。杆菌)。其中15% （N= 16473）的患者自报为糖尿病。该组ESBL阳性感染的患病率较高（18%，N=384），并且在有不止一种分离微生物的病例中，38%的人观察到ESBL阳性。光秃念珠菌在糖尿病患者中更为普遍。结论本组病例以18 ~ 50岁女性居多，以大肠杆菌为主。糖尿病患者esbl阳性微生物引起的感染发生率较高，尤其是混合性感染。此外，光滑假丝酵母在该组中更为普遍。研究结果表明，主要尿路感染病原体的流行率与近年来报道的相似。这项研究强调了定期更新流行病学数据以改善这些感染的临床管理策略的重要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Clinical chemistry 医学-医学实验技术

CiteScore

11.30

自引率

4.30%

发文量

212

审稿时长

1.7 months

期刊介绍： Clinical Chemistry is a peer-reviewed scientific journal that is the premier publication for the science and practice of clinical laboratory medicine. It was established in 1955 and is associated with the Association for Diagnostics & Laboratory Medicine (ADLM). The journal focuses on laboratory diagnosis and management of patients, and has expanded to include other clinical laboratory disciplines such as genomics, hematology, microbiology, and toxicology. It also publishes articles relevant to clinical specialties including cardiology, endocrinology, gastroenterology, genetics, immunology, infectious diseases, maternal-fetal medicine, neurology, nutrition, oncology, and pediatrics. In addition to original research, editorials, and reviews, Clinical Chemistry features recurring sections such as clinical case studies, perspectives, podcasts, and Q&A articles. It has the highest impact factor among journals of clinical chemistry, laboratory medicine, pathology, analytical chemistry, transfusion medicine, and clinical microbiology. The journal is indexed in databases such as MEDLINE and Web of Science.