Implementation of a fully biodegradable and biomimetic epicardial patch providing synergic physico-chemical, mechanical and electrical cues for myocardial infarction therapy

IF 6 2区医学 Q2 MATERIALS SCIENCE, BIOMATERIALS

Materials Science & Engineering C-Materials for Biological Applications Pub Date : 2025-09-24 DOI:10.1016/j.bioadv.2025.214523

Caterina Cristallini , Daniela Rossin , Niccoletta Barbani , Roberto Vanni , Massimiliano Labardi , Cheherazade Trouki , Silvia Burchielli , Claudia Kusmic , Domiziana Terlizzi , Francesca Sergi , Chiara Bulgheresi , Dawid Rossino , Erika Fiorino , Matteo Aubry , Marco Lo Iacono , Sadia Perveen , Giorgia Scarpellino , Luca Munaron , Sara Amorim , Ricardo A. Pires , Claudia Giachino

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引用次数: 0

Abstract

The intrinsic limitation of myocardial tissue to self-repair after damage underscores the need for innovative approaches in addressing cardiac tissue damage post-myocardial infarction (MI). We aimed to develop an acellular, bioartificial, microstructured and electroconductive patch (PGF) made of poly(lactic-co-glycolic acid) (PLGA), Gelatin, and 9-fluorenylmethoxycarbonyl-diphenylalanine (Fmoc-FF), to foster post-MI endogenous cardiac healing capabilities. The self-assembling semi-conductive peptide Fmoc-FF was introduced to reduce the electrical impedance of the polymer components while maintaining the complete biodegradation of the patch. Unexpectedly, the electroconductive component was found to increase the patch microstructure stability, improve cardiomyoblast elongation, augment stromal cell differentiation and sustain Human induced Pluripotent Stem Cell-derived Cardiomyocytes (hiPSC-CM) beating for at least 30 days. The main outcome was demonstrated in vivo, where epicardial implantation of the PGF patch in a rat model of ischaemia-reperfusion promoted significant cardiac tissue repair: this was evidenced by preservation of the myocardial tissue, reduced fibrosis, and recruitment of endogenous c-Kit+ cells. This newly implemented patch configuration promotes efficient myocardial healing, offering a promising therapeutic approach for infarcted patients.

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实现完全可生物降解的仿生心外膜贴片，为心肌梗死治疗提供物理化学、机械和电的协同提示。

心肌组织损伤后自我修复的内在局限性强调了解决心肌梗死后心肌组织损伤的创新方法的必要性。我们的目标是开发一种由聚乳酸-羟基乙酸（PLGA）、明胶和9-氟酰甲氧羰基-二苯丙氨酸（Fmoc-FF）制成的无细胞、生物人工、微结构和导电贴片（PGF），以促进心肌梗死后内源性心脏愈合能力。引入自组装半导电肽Fmoc-FF来降低聚合物组分的电阻抗，同时保持贴片的完全生物降解。出乎意料的是，导电成分被发现增加了贴片微观结构的稳定性，改善了成心肌细胞的延伸，增强了基质细胞的分化，并维持了人类诱导多能干细胞来源的心肌细胞（hiPSC-CM）至少30天的跳动。主要结果在体内得到证实，在缺血-再灌注大鼠模型中，心外膜植入PGF贴片促进了显著的心脏组织修复：心肌组织保存、纤维化减少和内源性c-Kit+细胞募集证明了这一点。这种新实施的贴片结构促进了有效的心肌愈合，为梗死患者提供了一种有希望的治疗方法。

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来源期刊

Materials Science & Engineering C-Materials for Biological Applications 工程技术-材料科学：生物材料

CiteScore

17.80

自引率

0.00%

发文量

501

审稿时长

27 days

期刊介绍： Biomaterials Advances, previously known as Materials Science and Engineering: C-Materials for Biological Applications (P-ISSN: 0928-4931, E-ISSN: 1873-0191). Includes topics at the interface of the biomedical sciences and materials engineering. These topics include: • Bioinspired and biomimetic materials for medical applications • Materials of biological origin for medical applications • Materials for "active" medical applications • Self-assembling and self-healing materials for medical applications • "Smart" (i.e., stimulus-response) materials for medical applications • Ceramic, metallic, polymeric, and composite materials for medical applications • Materials for in vivo sensing • Materials for in vivo imaging • Materials for delivery of pharmacologic agents and vaccines • Novel approaches for characterizing and modeling materials for medical applications Manuscripts on biological topics without a materials science component, or manuscripts on materials science without biological applications, will not be considered for publication in Materials Science and Engineering C. New submissions are first assessed for language, scope and originality (plagiarism check) and can be desk rejected before review if they need English language improvements, are out of scope or present excessive duplication with published sources. Biomaterials Advances sits within Elsevier''s biomaterials science portfolio alongside Biomaterials, Materials Today Bio and Biomaterials and Biosystems. As part of the broader Materials Today family, Biomaterials Advances offers authors rigorous peer review, rapid decisions, and high visibility. We look forward to receiving your submissions!