Histological, Immunohistochemical, and Biochemical Evaluation of the Effect of Berberine on Ovarian Hyperstimulation Syndrome in Rats

IF 1.5 4区 医学 Q3 OBSTETRICS & GYNECOLOGY
Filiz Yilmaz, Orkun Ilgen, Alper Mankan, Bayram Yilmaz, Ercan Ayaz, Sefa Kurt
{"title":"Histological, Immunohistochemical, and Biochemical Evaluation of the Effect of Berberine on Ovarian Hyperstimulation Syndrome in Rats","authors":"Filiz Yilmaz,&nbsp;Orkun Ilgen,&nbsp;Alper Mankan,&nbsp;Bayram Yilmaz,&nbsp;Ercan Ayaz,&nbsp;Sefa Kurt","doi":"10.1111/jog.70088","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Aim</h3>\n \n <p>OHSS is a potentially fatal condition. We aimed to investigate the effects of berberine administration on folliculogenesis and VEGF, TGF-α, IL-1β, and IL-6 levels in the OHSS model.</p>\n </section>\n \n <section>\n \n <h3> Method</h3>\n \n <p>A total of 28 female immature Wistar albino rats were divided into four groups: Sham group, Berberine group, OHSS group, and OHSS + Bb group. An OHSS model was created in which 10 IU PMSG was administered for four consecutive days and 30 IU hCG on Day 5. On Day 7, all subjects were sacrificed. Body weight, ovarian weight, histologic evaluation of ovaries, biochemical evaluation of TGF-α, IL-1β, and IL-6 expressions, and VEGF immunohistochemical analysis were performed.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Body weight, ovarian weight, follicular cell degeneration, vascular congestion, edema, and inflammation parameters were highest in the OHSS group (<i>p</i> &lt; 0.05). In the OHSS + Bb group, primary follicles and secondary follicles increased, while corpus luteum, atretic follicles, TGF-α, IL-1β, and IL-6 levels, and VEGF expressions decreased compared to the OHSS group (<i>p</i> &lt; 0.05). No statistical difference was observed between the control group and the OHSS + Bb group.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>In the present study, we demonstrated that berberine has the potential to prevent the development of OHSS in animal models. This finding suggests that further investigation into the use of berberine in human studies could provide valuable insights and inform the development of effective treatment strategies for OHSS. Consequently, the risk of OHSS can be reduced by administering berberine to patients undergoing the controlled ovarian hyperstimulation protocol. Therefore, the life risk of patients due to OHSS may be eliminated.</p>\n </section>\n </div>","PeriodicalId":16593,"journal":{"name":"Journal of Obstetrics and Gynaecology Research","volume":"51 10","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Obstetrics and Gynaecology Research","FirstCategoryId":"3","ListUrlMain":"https://obgyn.onlinelibrary.wiley.com/doi/10.1111/jog.70088","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"OBSTETRICS & GYNECOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Aim

OHSS is a potentially fatal condition. We aimed to investigate the effects of berberine administration on folliculogenesis and VEGF, TGF-α, IL-1β, and IL-6 levels in the OHSS model.

Method

A total of 28 female immature Wistar albino rats were divided into four groups: Sham group, Berberine group, OHSS group, and OHSS + Bb group. An OHSS model was created in which 10 IU PMSG was administered for four consecutive days and 30 IU hCG on Day 5. On Day 7, all subjects were sacrificed. Body weight, ovarian weight, histologic evaluation of ovaries, biochemical evaluation of TGF-α, IL-1β, and IL-6 expressions, and VEGF immunohistochemical analysis were performed.

Results

Body weight, ovarian weight, follicular cell degeneration, vascular congestion, edema, and inflammation parameters were highest in the OHSS group (p < 0.05). In the OHSS + Bb group, primary follicles and secondary follicles increased, while corpus luteum, atretic follicles, TGF-α, IL-1β, and IL-6 levels, and VEGF expressions decreased compared to the OHSS group (p < 0.05). No statistical difference was observed between the control group and the OHSS + Bb group.

Conclusion

In the present study, we demonstrated that berberine has the potential to prevent the development of OHSS in animal models. This finding suggests that further investigation into the use of berberine in human studies could provide valuable insights and inform the development of effective treatment strategies for OHSS. Consequently, the risk of OHSS can be reduced by administering berberine to patients undergoing the controlled ovarian hyperstimulation protocol. Therefore, the life risk of patients due to OHSS may be eliminated.

Abstract Image

小檗碱对卵巢过度刺激综合征大鼠影响的组织学、免疫组织化学和生化评价。
目的:OHSS是一种潜在的致命疾病。我们的目的是研究黄连素给药对OHSS模型卵泡生成和VEGF、TGF-α、IL-1β和IL-6水平的影响。方法:将雌性未成熟Wistar白化大鼠28只分为Sham组、Berberine组、OHSS组、OHSS + Bb组。建立OHSS模型,连续4天给予10 IU PMSG,第5天给予30 IU hCG。第7天,所有受试者均被处死。进行体重、卵巢重量、卵巢组织学评价、生化评价TGF-α、IL-1β、IL-6表达及VEGF免疫组化分析。结果:OHSS组大鼠体重、卵巢重量、卵泡细胞变性、血管充血、水肿和炎症参数最高(p)。结论:在本研究中,我们在动物模型中证明了小檗碱具有预防OHSS发展的潜力。这一发现表明,进一步研究小檗碱在人体研究中的使用可以提供有价值的见解,并为OHSS的有效治疗策略的发展提供信息。因此,可以通过给接受控制卵巢过度刺激方案的患者施用小檗碱来降低OHSS的风险。因此,可以消除OHSS患者的生命风险。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
3.10
自引率
0.00%
发文量
376
审稿时长
3-6 weeks
期刊介绍: The Journal of Obstetrics and Gynaecology Research is the official Journal of the Asia and Oceania Federation of Obstetrics and Gynecology and of the Japan Society of Obstetrics and Gynecology, and aims to provide a medium for the publication of articles in the fields of obstetrics and gynecology. The Journal publishes original research articles, case reports, review articles and letters to the editor. The Journal will give publication priority to original research articles over case reports. Accepted papers become the exclusive licence of the Journal. Manuscripts are peer reviewed by at least two referees and/or Associate Editors expert in the field of the submitted paper.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信