Autonomous Recovery of Wheat Spikelet Development following Cold Stress Arrest Mediated by Modulation of Sucrose Degradation and IAA/ABA Homeostasis.

IF 5.7 2区 生物学 Q1 PLANT SCIENCES
Hui Su, Yujian Yang, Yamiao Zhang, Yadong Wang, Ashley Jones, Jinpeng Li, Youhong Song
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引用次数: 0

Abstract

Wheat rapidly induces complex metabolic reactions in response to cold stress, yet the physiological mechanisms governing its natural recovery process remain poorly understood. In a two-year pot experiment, we examined recovery dynamics of the wheat cultivar Zhengmai 366 during booting stage under control (CK, 10/10 °C), chilling (CS, 10/2 °C), and freezing (FS, 10/-2 °C) treatments. Following stress relief, we performed comprehensive analyses on spikelet morphology, physiology, transcriptomes, and metabolomes. Spikelet development was consistently delayed in both post-cold recovery scenarios, with an irreversible damage due to cellular breakdown during FS recovery. Physiological investigations demonstrated that antioxidant enzyme activities, sucrose, hexose, and proline concentration restored to normal levels after CS recovery, but remained suppressed after FS recovery. Furthermore, a progressive increase in IAA levels and a progressive decline in ABA levels concurred during the CS recovery, which may facilitate the resumption of spikelet development. Machine learning highlighted sucrose content and the IAA/ABA ratio as primary predictors of grain number. Multi-omics integration further confirmed that the recovery is determined by sucrose-hexose conversion efficiency and hormonal balance. Collectively, this study revealed that wheat recovery from cold is mediated by coordinated carbon metabolism and hormonal homeostasis. This provided valuable insights toward improving cold tolerance in wheat production.

蔗糖降解和IAA/ABA平衡调控下小麦小穗发育在低温胁迫下的自主恢复
小麦在冷胁迫下快速诱导复杂的代谢反应,但控制其自然恢复过程的生理机制尚不清楚。通过2年盆栽试验,研究了小麦品种郑麦 366孕穗期在对照(CK, 10/10 °C)、冷藏(CS, 10/2 °C)和冷冻(FS, 10/-2 °C)处理下的恢复动态。胁迫解除后,我们对小穗形态、生理、转录组和代谢组进行了综合分析。在低温恢复后的两种情况下,小穗发育都持续延迟,在低温恢复过程中由于细胞破坏造成了不可逆的损伤。生理研究表明,抗氧化酶活性、蔗糖、己糖和脯氨酸浓度在CS恢复后恢复到正常水平,但在FS恢复后仍然受到抑制。此外,在CS恢复过程中,IAA水平的逐渐增加和ABA水平的逐渐下降同时发生,这可能有助于小穗发育的恢复。机器学习强调蔗糖含量和IAA/ABA比是籽粒数的主要预测因子。多组学整合进一步证实,恢复是由蔗糖-己糖转化效率和激素平衡决定的。综上所述,本研究揭示了小麦从寒冷中恢复是由碳代谢和激素稳态协调介导的。这为提高小麦的耐寒性提供了有价值的见解。
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来源期刊
Journal of Experimental Botany
Journal of Experimental Botany 生物-植物科学
CiteScore
12.30
自引率
4.30%
发文量
450
审稿时长
1.9 months
期刊介绍: The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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