Influence of the antidiabetic drug metformin on the aquatic crustacean Daphnia magna.

IF 2 4区医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL

Journal of applied biomedicine Pub Date : 2025-09-01 Epub Date: 2025-08-21 DOI:10.32725/jab.2025.011

Martina Poncarova, Sarka Klementova, Michal Sorf

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引用次数: 0

Abstract

Background: The antidiabetic drug metformin has been repeatedly detected in surface waters worldwide. This study investigates the effects of the environmentally relevant concentration of metformin on a non-target aquatic organism - a freshwater crustacean, Daphnia magna, with an emphasis on the stress response of daphnids and the long-term effects on their consecutive generations.

Methods: The chronic toxicity test and the consecutive generations test were inspired by the OECD method. The total antioxidant capacity (Trolox equivalent - TEAC), superoxide dismutase (SOD) activity, and catalase (CAT) activity were related to the protein content in the tested daphnids.

Results: Elevated antioxidant activities were revealed in daphnids exposed to metformin in comparison to the control group (1.9 × for TEAC, 1.7 × for SOD; 1.3 × for CAT). Furthermore, diminished body sizes and malformations in the digestive system, spine and carapace were detected in newborn juveniles in the second and third generations exposed to metformin.

Conclusion: Long-term exposure to metformin in environmentally relevant concentrations led to a significant detrimental reaction in aquatic crustaceans.

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降糖药二甲双胍对水生甲壳动物大水蚤的影响。

背景：降糖药二甲双胍在世界各地地表水中多次检出。本研究研究了环境相关浓度二甲双胍对非靶水生生物淡水甲壳类大水蚤的影响，重点研究了水蚤的应激反应及其对其后代的长期影响。方法：采用OECD法进行慢性毒性试验和连续代试验。水蚤总抗氧化能力（Trolox equivalent - TEAC）、超氧化物歧化酶（SOD）活性和过氧化氢酶（CAT）活性与蛋白质含量有关。结果：与对照组相比，暴露于二甲双胍的水蚤抗氧化活性升高（TEAC 1.9倍，SOD 1.7倍，CAT 1.3倍）。此外，在暴露于二甲双胍的第二代和第三代新生幼鱼中发现了体型缩小和消化系统、脊柱和甲壳的畸形。结论：长期暴露于环境相关浓度的二甲双胍会导致水生甲壳类动物发生显著的有害反应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of applied biomedicine PHARMACOLOGY & PHARMACY-

CiteScore

2.40

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Applied Biomedicine promotes translation of basic biomedical research into clinical investigation, conversion of clinical evidence into practice in all medical fields, and publication of new ideas for conquering human health problems across disciplines. Providing a unique perspective, this international journal publishes peer-reviewed original papers and reviews offering a sensible transfer of basic research to applied clinical medicine. Journal of Applied Biomedicine covers the latest developments in various fields of biomedicine with special attention to cardiology and cardiovascular diseases, genetics, immunology, environmental health, toxicology, neurology and oncology as well as multidisciplinary studies. The views of experts on current advances in nanotechnology and molecular/cell biology will be also considered for publication as long as they have a direct clinical impact on human health. The journal does not accept basic science research or research without significant clinical implications. Manuscripts with innovative ideas and approaches that bridge different fields and show clear perspectives for clinical applications are considered with top priority.