Structural characteristics of two pectic polysaccharides from Avicennia marina fruit.

IF 2.5 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2025-09-22 DOI:10.1016/j.carres.2025.109679

Huaqun Chen, Wennian Li, Chunyu Xiao, Xiaotao Hou, Kefeng Wu, Longyan Zhao, Qingxia Yuan

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引用次数: 0

Abstract

Pectic polysaccharides in A. marina fruit exhibit excellent prebiotic effects and may be the key to treating constipation and dysentery; however, their detailed structures still need to be clarified. In this study, we obtained two pectic polysaccharide fractions (AMFPs-3-2 and AMFPs-3-4) from this fruit for the first time and comprehensively investigated their fine structures using chemical methods, GC-MS, and 1D and 2D-NMR. AMFPs-3-2 was identified as a type I rhamnogalacturonan (RG-I) with a backbone composed of →2)-α-Rhap-(1 → 4)-α-GalpA-(1→, and the side chains mainly consisted of arabinan with non-reducing ends of α/β-Araf. Meanwhile, AMFPs-3-4 was characterized as a homogalacturonan (HG) composed of galacturonic acid (GalpA) and methyl-esterified GalpA, with GalpA, (un)saturated GalpA and methyl-esterified (un)saturated GalpA as non-reducing ends, and α/β-GalpA or methyl-esterified α/β-GalpA as reducing ends. These findings demonstrate that different types of pectic polysaccharides from A. marina fruit can be effectively separated and distinguished. The elucidation of these clear structural features provides a solid structural basis for further investigating the pharmacological activity and structure-activity relationship of polysaccharides from A. marina fruit.

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海棠果实两种果胶多糖的结构特征。

果胶多糖具有良好的益生元作用，可能是治疗便秘和痢疾的关键；然而，它们的详细结构仍然需要澄清。本研究首次从该果实中分离得到两个果胶多糖组分（AMFPs-3-2和AMFPs-3-4），并利用化学方法、GC-MS、1D和2D-NMR对其精细结构进行了全面研究。amfp -3-2被鉴定为RG-I型鼠李糖半乳糖酸（RG-I），其主链由→2)-α- rhap -(1→4)-α- galpa -（1→）组成，侧链主要由α/β-Araf非还原端组成的arabinan组成。同时，amfp -3-4表征为半乳糖醛酸（GalpA）和甲基化GalpA组成的均半乳糖醛酸（HG），以GalpA、（非）饱和GalpA和甲基化（非）饱和GalpA为非还原端，α/β-GalpA或甲基化α/β-GalpA为还原端。结果表明，该方法可以有效地分离和区分不同类型的果胶多糖。这些明确的结构特征的阐明，为进一步研究海青果多糖的药理活性和构效关系提供了坚实的结构基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".