MOF-based ratiometric fluorescent biosensors for selective and sensitive detection of hydrogen sulfide in food products

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-09-30 DOI:10.1007/s00604-025-07564-5

Yang Shen, Rou Chen, Yun Wang, Yanjun Qiu, Yuting Zhang, Xiaoli Wang, Nandi Zhou

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Abstract

Hydrogen a metal–organic framework (MOF)-based ratiometric fluorescent biosensor (Uio-66-N₃@Cy3) was developed for the determination of hydrogen sulfide (H₂S). The sensor combines the high specificity of the H₂S-azide reaction with the inherent self-calibration capability of MOF-based dual-emission signals for efficient detection. The biosensor operates via H₂S-selective azide-to-amine conversion, which suppresses photoinduced electron transfer to simultaneously restore the MOF's intrinsic fluorescence while maintaining Cy3's stable fluorescence, enabling ratiometric detection. The biosensor exhibits a dual-linear response across 0.5–100 nM and 0.5–4.0 μM, with detection limits as low as 36 pM and 27 nM, respectively. Furthermore, the biosensor shows exceptional selectivity against 18 potential interferents and long-term stability (RSD < 3.5% over 14 days). Importantly, the ratiometric mechanism enables robust performance in complex food samples (beer and milk), yielding 99.74–103.00% recoveries with minimal variance. Unlike intensity-based probes, this system provides intrinsic calibration through simultaneous dual-channel detection, making it ideal for real-world applications. Our work not only delivers a superior H₂S sensor but also establishes a generalizable platform for ratiometric detection of diverse contaminants in food systems.

Graphical Abstract

查看原文本刊更多论文

基于mof的比例荧光生物传感器用于食品中硫化氢的选择性和灵敏检测。

氢是一种基于金属有机骨架（MOF）的比例荧光生物传感器（Uio-66-N3@Cy3），用于硫化氢（H2S）的测定。该传感器结合了h2s -叠氮化反应的高特异性和mof双发射信号固有的自校准能力，实现了高效检测。该生物传感器通过h2s选择性叠氮化物到胺的转化，抑制光诱导的电子转移，同时恢复MOF的固有荧光，同时保持Cy3的稳定荧光，从而实现比率检测。该传感器在0.5 ~ 100 nM和0.5 ~ 4.0 μM范围内具有双线性响应，检测限分别低至36 pM和27 nM。此外，该生物传感器对18种潜在干扰具有出色的选择性和长期稳定性（RSD 2S传感器），同时也为食品系统中各种污染物的比例检测建立了一个通用平台。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.