Geometry-Guided Osteogenesis in Bone-on-a-Chip Systems Using Triply Periodic Minimal Surface Scaffolds.

IF 9.6 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Donggyu Kim, Giheon Ha, Minseok Kim, Minjin Kwak, Han-Jun Kim, Junmin Lee
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引用次数: 0

Abstract

The interplay between scaffold geometry and mechanical cues is critical in regulating osteogenesis within engineered bone microenvironments. To better mimic native bone physiology and improve regeneration strategies, it is essential to integrate precise topological control with physiologically relevant flow. Here, a bone-on-a-chip (BoC) system coupled with triply periodic minimal surface (TPMS)-based 3D scaffolds is presented to investigate how geometric parameters-pore shape and solidity-govern osteogenic responses under dynamic perfusion. Using Gyroid and Schwarz diamond TPMS architectures, scaffolds with controlled pore geometries are created to modulate wall shear stress (WSS). Under flow conditions in the BoC system, pre-osteoblasts exhibit geometry-dependent behaviors in terms of infiltration, alkaline phosphatase activity, calcium deposition, and collagen formation. Scaffolds with intermediate solidity and curvature optimize WSS distribution and significantly enhance osteogenic differentiation. Additionally, a critical pore size threshold is identified beyond which flow-mediated signaling is attenuated, highlighting the importance of geometric precision. The results demonstrate the synergistic role of scaffold topology and interstitial flow in directing osteogenesis. This integrated platform provides a versatile tool for studying bone mechanobiology and offers a promising strategy for designing biomimetic scaffolds in regenerative medicine and bone tissue engineering.

三周期最小表面支架在骨芯片系统中的几何引导成骨。
支架几何和机械线索之间的相互作用在工程骨微环境中调节成骨至关重要。为了更好地模拟天然骨生理和改善再生策略,必须将精确的拓扑控制与生理相关的流动结合起来。本研究采用骨片(BoC)系统与基于三周期最小表面(TPMS)的3D支架相结合,研究几何参数(孔隙形状和固体度)如何影响动态灌注下的成骨反应。利用Gyroid和Schwarz金刚石TPMS结构,创建了具有可控孔隙几何形状的支架来调节壁面剪切应力(WSS)。在BoC系统的流动条件下,成骨前细胞在浸润、碱性磷酸酶活性、钙沉积和胶原形成方面表现出几何依赖性行为。具有中等硬度和曲率的支架优化WSS分布,显著增强成骨分化。此外,还确定了一个临界孔径阈值,超过该阈值,流介导的信号就会减弱,从而突出了几何精度的重要性。结果表明支架拓扑结构和间质流动在指导成骨过程中具有协同作用。该集成平台为骨力学生物学研究提供了一个多功能的工具,为再生医学和骨组织工程仿生支架的设计提供了一个有前途的策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advanced Healthcare Materials
Advanced Healthcare Materials 工程技术-生物材料
CiteScore
14.40
自引率
3.00%
发文量
600
审稿时长
1.8 months
期刊介绍: Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.
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