Nitric Oxide-Enhanced Chitosan-Based Nanoparticles for Dual-Mode Photothermal and Photodynamic Therapy in Infected Wounds.

IF 9.6 2区 医学 Q1 ENGINEERING, BIOMEDICAL
Yueting Li, Siting Du, Yanzhen Feng, Xiaopeng Zhou, Kaiting Zhao, Jianfeng Wang, Quan Zhou, Yaru Shi, Ayesha Younas, Yonghui Shen, Shuanghu Wang
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引用次数: 0

Abstract

Bacterial infections pose a significant challenge to global health, as conventional treatments frequently cause side effects and resistance. Phototherapy, encompassing photothermal therapy (PTT) and photodynamic therapy (PDT), emerges as a viable alternative, eradicating bacteria through heat and reactive oxygen species (ROS). However, excessive heat and ROS can exacerbate local inflammation and comlicate healing. To address these challenges, we developed a novel chitosan-based nanoparticle (NPs) encapsulating S-nitrosoglutathione (GSNO) and indocyanine green (ICG), termed as GSNO/ICG@NPs. This biocompatible nanosystem integrates the triple synergistic effects of nitric oxide (NO) release, PTT, and PDT. The stable and uniformly shaped GSNO/ICG@NPs were synthesized via a charge-driven self-assembly method with efficient loading of GSNO and ICG. Upon irradiation with an 808 nm near-infrared (NIR) laser, GSNO/ICG@NPs rapidly generate heat and 1O2, effectively eliminating bacteria. Simultaneously, the photothermal effect releases NO from GSNO, modulates inflammation, promotes neovascularization, and supports tissue repair. In vitro and in vivo studies demonstrated that GSNO/ICG@NPs significantly enhance antibacterial activity, reduce inflammation, and promote angiogenesis. It effectively eradicated biofilms and accelerated wound healing in a Staphylococcus aureus-infected mouse skin model. These findings highlight GSNO/ICG@NPs as a promising alternative to antibiotics for treating infected wounds via synergistic therapy involving balanced NO release, PTT, and PDT.

一氧化氮增强壳聚糖纳米颗粒用于感染伤口的双模光热和光动力治疗。
细菌感染对全球健康构成重大挑战,因为传统治疗方法经常产生副作用和耐药性。光疗,包括光热疗法(PTT)和光动力疗法(PDT),作为一种可行的替代方案出现,通过热和活性氧(ROS)来消灭细菌。然而,过热和活性氧可加剧局部炎症和复杂的愈合。为了解决这些挑战,我们开发了一种新型的壳聚糖基纳米颗粒(NPs),该纳米颗粒封装了s -亚硝基谷胱甘肽(GSNO)和吲哚菁绿(ICG),称为GSNO/ICG@NPs。这种生物相容性纳米系统整合了一氧化氮(NO)释放、PTT和PDT的三重协同效应。采用电荷驱动的自组装方法,高效负载GSNO和ICG,合成了形状均匀稳定的GSNO/ICG@NPs。在808 nm近红外(NIR)激光照射下,GSNO/ICG@NPs迅速产生热量和10o2,有效杀灭细菌。同时,光热效应从GSNO中释放NO,调节炎症,促进新生血管形成,支持组织修复。体外和体内研究表明,GSNO/ICG@NPs显著增强抗菌活性,减少炎症,促进血管生成。在金黄色葡萄球菌感染的小鼠皮肤模型中,它有效地根除生物膜并加速伤口愈合。这些发现突出了GSNO/ICG@NPs作为一种有希望的替代抗生素治疗感染伤口通过协同治疗包括平衡NO释放,PTT和PDT。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Advanced Healthcare Materials
Advanced Healthcare Materials 工程技术-生物材料
CiteScore
14.40
自引率
3.00%
发文量
600
审稿时长
1.8 months
期刊介绍: Advanced Healthcare Materials, a distinguished member of the esteemed Advanced portfolio, has been dedicated to disseminating cutting-edge research on materials, devices, and technologies for enhancing human well-being for over ten years. As a comprehensive journal, it encompasses a wide range of disciplines such as biomaterials, biointerfaces, nanomedicine and nanotechnology, tissue engineering, and regenerative medicine.
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