Water-Soluble Squaraine-Based NIR Chromofluorogenic Probe for HClO/ClO^- Detection in Environmental Waters and Live-Cell Bioimaging.

IF 3.7 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemistry - A European Journal Pub Date : 2025-10-01 DOI:10.1002/chem.202502401

Huifang Li, Wanying Li, Yiru Tang, Xinsen He, Ying Xiong, Yimou Gong, Ji Lu, Xianchao Pan, Jun Wang, Na Hao, Lin Yang

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引用次数: 0

Abstract

Hypochlorous acid/hypochlorite (HClO/ClO^-), as critical biological reactive oxygen species (ROS), plays essential roles in numerous physiological and pathological processes. To enable sensitive detection of HClO/ClO^- in environmental water samples and living cells, a near-infrared (NIR) water-soluble squaraine-based chromofluorogenic probe SO₃SQ was developed. SO₃SQ exhibits good water solubility, NIR detection wavelength (696 nm/725 nm), high selectivity, rapid response time (within seconds), visible colorimetric response, and minimal cytotoxicity. Furthermore, SO₃SQ achieved quantitative HClO/ClO^- detection in environmental water samples and realized real-time fluorescence imaging of exogenous/endogenous HClO/ClO^- in HepG2 cells. This work provides new ideas for the future design of NIR water-soluble HClO/ClO^- probes, while expanding the application of squaraine dyes as HClO/ClO^- probes.

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水溶性角鲨胺基近红外荧光探针用于环境水体中HClO/ClO-的检测和活细胞生物成像。

次氯酸/次氯酸盐（HClO/ClO-）是一种重要的生物活性氧（ROS），在许多生理和病理过程中起着重要作用。为了灵敏地检测环境水样和活细胞中的HClO/ClO-，研制了一种近红外（NIR）水溶性角鲨碱荧光探针SO3SQ。SO3SQ具有良好的水溶性，近红外检测波长（696 nm/725 nm），高选择性，快速响应时间（秒内），可见比色响应和最小的细胞毒性。SO3SQ实现了环境水样中HClO/ClO-的定量检测，实现了HepG2细胞外源性/内源性HClO/ClO-的实时荧光成像。本研究为未来近红外水溶性HClO/ClO-探针的设计提供了新的思路，同时也拓展了方碱染料作为HClO/ClO-探针的应用。

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来源期刊

Chemistry - A European Journal 化学-化学综合

CiteScore

7.90

自引率

4.70%

发文量

1808

审稿时长

1.8 months

期刊介绍： Chemistry—A European Journal is a truly international journal with top quality contributions (2018 ISI Impact Factor: 5.16). It publishes a wide range of outstanding Reviews, Minireviews, Concepts, Full Papers, and Communications from all areas of chemistry and related fields. Based in Europe Chemistry—A European Journal provides an excellent platform for increasing the visibility of European chemistry as well as for featuring the best research from authors from around the world. All manuscripts are peer-reviewed, and electronic processing ensures accurate reproduction of text and data, plus short publication times. The Concepts section provides nonspecialist readers with a useful conceptual guide to unfamiliar areas and experts with new angles on familiar problems. Chemistry—A European Journal is published on behalf of ChemPubSoc Europe, a group of 16 national chemical societies from within Europe, and supported by the Asian Chemical Editorial Societies. The ChemPubSoc Europe family comprises: Angewandte Chemie, Chemistry—A European Journal, European Journal of Organic Chemistry, European Journal of Inorganic Chemistry, ChemPhysChem, ChemBioChem, ChemMedChem, ChemCatChem, ChemSusChem, ChemPlusChem, ChemElectroChem, and ChemistryOpen.