Functional insights into drought-tolerant PGPR: impacts on physiological responses and yield performance of direct-seeded rice under water stress

IF 2.1 3区生物学 Q2 MULTIDISCIPLINARY SCIENCES

The Science of Nature Pub Date : 2025-10-02 DOI:10.1007/s00114-025-02025-8

Faiza Javed, Sumera Iqbal, Muhammad Shahbaz Farooq, Khajista Jabeen, Noshin Ilyas, Abid Majeed

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Abstract

Drought stress poses a significant threat to rice productivity, particularly when rice is cultivated through the direct seeded system (DSR). This paper aimed to isolate and characterize drought-tolerant plant growth-promoting rhizobacteria (PGPR) from rice rhizospheric soils in arid and semi-arid areas of Pakistan and assess their efficacy in improving rice growth under drought conditions. Six isolates exhibited PGPR traits such as phosphate solubilization, indole 3-acetic acid production, ACC deaminase activity, siderophore production, and osmolyte accumulation. Pot experiments under drought stress (60% field capacity) demonstrated that inoculation with PGPR enhanced the germination rate, chlorophyll level, water status, membrane stability, and biochemical parameters in rice exceptionally. Among these, isolate NRC13, identified as Bacillus velezensis, showed the greatest efficacy in enhancing physiological, biochemical, and yield characteristics, including grain yield and 1000-grain weight under drought stress. This study provides the first evidence of Bacillus velezensis NRC13’s application in direct-seeded rice under drought conditions in Pakistan, demonstrating the potential of this novel bio-inoculant to increase rice productivity and resilience in water-deficient settings.

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抗旱PGPR的功能研究：对水分胁迫下直播水稻生理反应和产量的影响

干旱胁迫对水稻生产力构成重大威胁，特别是当水稻通过直接播种系统（DSR）种植时。本文旨在从巴基斯坦干旱和半干旱地区的水稻根际土壤中分离到耐旱植物促生根瘤菌（PGPR），并对其进行鉴定，评价其在干旱条件下促进水稻生长的效果。6株菌株表现出磷酸增溶、吲哚3-乙酸生成、ACC脱氨酶活性、铁载体生成和渗透物积累等PGPR性状。干旱胁迫下盆栽试验（60%田间容量）表明，接种PGPR显著提高了水稻的发芽率、叶绿素水平、水分状况、膜稳定性和生化参数。其中，鉴定为velezensis芽孢杆菌的NRC13在干旱胁迫下提高籽粒产量和千粒重等生理生化和产量特性的效果最大。这项研究首次证明了velezensis NRC13在巴基斯坦干旱条件下在直接播种水稻上的应用，证明了这种新型生物接种剂在缺水环境下提高水稻生产力和抗逆性的潜力。

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来源期刊

The Science of Nature 综合性期刊-综合性期刊

CiteScore

3.40

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： The Science of Nature - Naturwissenschaften - is Springer''s flagship multidisciplinary science journal. The journal is dedicated to the fast publication and global dissemination of high-quality research and invites papers, which are of interest to the broader community in the biological sciences. Contributions from the chemical, geological, and physical sciences are welcome if contributing to questions of general biological significance. Particularly welcomed are contributions that bridge between traditionally isolated areas and attempt to increase the conceptual understanding of systems and processes that demand an interdisciplinary approach.