Structural characterization, immune activity, and action mechanisms of fractionated polysaccharides from Athyrium multidentatum (Doll.) Ching

IF 2.2 4区化学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Carbohydrate Chemistry Pub Date : 2025-07-24 DOI:10.1080/07328303.2025.2527564

Ji-yu Chen , Ji-wen Sheng , Ting Wang , Yu-jie Yang , Jie Yang , Hong-wei Li , Han-tong Xiu , Xuan-chen Lu , Dong-mei Liu

{"title":"Structural characterization, immune activity, and action mechanisms of fractionated polysaccharides from Athyrium multidentatum (Doll.) Ching","authors":"Ji-yu Chen , Ji-wen Sheng , Ting Wang , Yu-jie Yang , Jie Yang , Hong-wei Li , Han-tong Xiu , Xuan-chen Lu , Dong-mei Liu","doi":"10.1080/07328303.2025.2527564","DOIUrl":null,"url":null,"abstract":"<div><div>A water-soluble polysaccharide (AFP) was extracted from <em>Athyrium Multidentatum</em> (Doll.) Ching by water extraction and alcohol precipitation method and purified by column chromatography. AFP was characterized by Fourier transform infrared (FT–IR) spectroscopy, ultraviolet-visible (UV–Vis) spectroscopy, circular dichroism (CD) spectroscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), Congo red (CR) binding assay, monosaccharide composition and molecular weight analyses. 1D and 2D nuclear magnetic resonance (NMR) studies revealed two structural components in AFP, which are →4-β-D-Man<em>p</em>-(1→4)-β-D-Xyl<em>p</em>-(1→4)-β-D-Xyl<em>p</em>-(1→2)-β-D-Gal<em>p</em>-(1→ and β-D-Glc<em>p</em>-(1→4)-β-D-Glc<em>p</em>-(1→4)-β-D-Glc<em>p</em>-(1→2)-β-D-Gal<em>p</em>-(1→. AFP exhibited significant immunological activities in both normal and immunosuppressed mice. Dectin-2/SYK/MAPK/non-classical NF-κB signaling pathways might play a crucial role in the immunomodulatory actions of AFP. Current results provided an important basis for the development of AFP as a natural immunoregulator.</div></div>","PeriodicalId":15311,"journal":{"name":"Journal of Carbohydrate Chemistry","volume":"44 4","pages":"Pages 196-230"},"PeriodicalIF":2.2000,"publicationDate":"2025-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Carbohydrate Chemistry","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/org/science/article/pii/S0732830325000096","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

A water-soluble polysaccharide (AFP) was extracted from Athyrium Multidentatum (Doll.) Ching by water extraction and alcohol precipitation method and purified by column chromatography. AFP was characterized by Fourier transform infrared (FT–IR) spectroscopy, ultraviolet-visible (UV–Vis) spectroscopy, circular dichroism (CD) spectroscopy, scanning electron microscopy (SEM), atomic force microscopy (AFM), Congo red (CR) binding assay, monosaccharide composition and molecular weight analyses. 1D and 2D nuclear magnetic resonance (NMR) studies revealed two structural components in AFP, which are →4-β-D-Manp-(1→4)-β-D-Xylp-(1→4)-β-D-Xylp-(1→2)-β-D-Galp-(1→ and β-D-Glcp-(1→4)-β-D-Glcp-(1→4)-β-D-Glcp-(1→2)-β-D-Galp-(1→. AFP exhibited significant immunological activities in both normal and immunosuppressed mice. Dectin-2/SYK/MAPK/non-classical NF-κB signaling pathways might play a crucial role in the immunomodulatory actions of AFP. Current results provided an important basis for the development of AFP as a natural immunoregulator.

查看原文本刊更多论文

多齿胸草多糖的结构特征、免疫活性和作用机制京

从多齿胸草（Athyrium Multidentatum, Doll）中提取水溶性多糖（AFP）。经水提醇沉法纯化，柱层析法纯化。采用傅里叶变换红外（FT-IR）光谱、紫外-可见（UV-Vis）光谱、圆二色（CD）光谱、扫描电镜（SEM）、原子力显微镜（AFM）、刚果红（CR）结合实验、单糖组成和分子量分析对AFP进行了表征。1D和2D核磁共振（NMR）研究揭示了AFP的两种结构成分：→4-β- d - manp -(1→4)-β- d - xylp -(1→4)-β- d - xylp -(1→2)-β- d - galp -(1→4)-β-D-Glcp-(1→4)-β-D-Glcp-(1→2)-β- d - galp -(1→2)-β- d - galp -（1→）。AFP在正常小鼠和免疫抑制小鼠中均表现出显著的免疫活性。Dectin-2/SYK/MAPK/非经典NF-κB信号通路可能在AFP的免疫调节作用中发挥重要作用。目前的结果为AFP作为一种天然免疫调节剂的开发提供了重要的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Carbohydrate Chemistry 化学-生化与分子生物学

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Carbohydrate Chemistry serves as an international forum for research advances involving the chemistry and biology of carbohydrates. The following aspects are considered to fall within the scope of this journal: -novel synthetic methods involving carbohydrates, oligosaccharides, and glycoconjugates- the use of chemical methods to address aspects of glycobiology- spectroscopic and crystallographic structure studies of carbohydrates- computational and molecular modeling studies- physicochemical studies involving carbohydrates and the chemistry and biochemistry of carbohydrate polymers.