An activity-based probe library for identifying promiscuous amide hydrolases

Abstract

A fluorogenic substrate library was developed to detect amide hydrolase activity in soil-derived chitin-degrading bacteria. Hit compounds were converted into pull-down probes for chemoproteomic enrichment, identifying previously unannotated proteins now linked to amide hydrolysis. This approach enables discovery of novel amide hydrolases with potential applications in environment, biomanufacturing, and medicine.