Honey bee nutritranscriptomics reveals key insights towards precision nutrition

IF 13 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Alexander McMenamin, Vincent Ricigliano
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引用次数: 0

Abstract

Introduction

Honey bees are essential managed pollinators faced by nutritional deficiencies which contributes to worldwide colony losses. The integration of nutrition science with genomics offers a powerful approach to optimize honey bee health by augmenting immune function and resilience to stressors through precision diet interventions.

Objectives

To uncover the impact of honey bee genetics on core physiological responses to natural and artificial diets.

Methods

Two honey bee genetic backgrounds (Russian and Pol-line) were fed diets consisting of sugar, pollen, and microalgae biomass from either Chlorella vulgaris or spirulina. Nutrigenetic variation was assessed by body weight measurements followed by transcriptome sequencing. Standard differential gene expression analysis and supervised machine learning algorithms were used to measure genotype-specific transcriptional responses to the diets.

Results

Known (i.e., vitellogenin and Jhe, hex70a) and novel (i.e., Glob1, slif and sad) biomarkers of nutritional health exhibited a significant genetics-by-diet interaction. Furthermore, Russian bees featured a contracted transcriptional response, and a transcriptomic signature of delayed behavioral maturation as compared to Pol-line bees, suggesting alternative nutrient assimilation strategies between the two genetic backgrounds. Regardless of diet, Russian bees had significantly lower abundance of a major viral pathogen (DWV). Lastly, higher expression of humoral and cellular immune genes was detected in microalgae-fed bees, though spirulina was significantly more immunogenic than Chlorella.

Conclusion

Genetic background plays a key role in shaping honey bee responses to nutrition. By identifying novel biomarkers of nutritional status, our results support the unique benefits of two microalgae species as functional feed additives. Furthermore, these results reveal the importance of genetics as a consideration when optimizing supplemental feed for an agricultural pollinator. These findings contribute towards the development of tailored nutrition strategies and artificial diets to improve honey bee health.

Abstract Image

蜜蜂营养转录组学揭示了精确营养的关键见解
蜜蜂是重要的传粉媒介,面临着营养缺乏,导致世界范围内的蜂群损失。营养科学与基因组学的整合提供了一种强大的方法,通过精确的饮食干预来增强免疫功能和对压力源的恢复力,从而优化蜜蜂的健康。目的揭示蜜蜂遗传对天然和人工饲料核心生理反应的影响。方法饲喂两种遗传背景的蜜蜂(俄罗斯蜜蜂和波兰蜜蜂),饲料中分别含有糖、花粉和来自小球藻或螺旋藻的微藻生物量。通过体重测量和转录组测序来评估营养遗传变异。标准差异基因表达分析和监督机器学习算法用于测量对饮食的基因型特异性转录反应。结果已知的(如卵黄原蛋白和Jhe, hex70a)和新的(如Glob1, slif和sad)营养健康生物标志物表现出显著的遗传-饮食相互作用。此外,与polline蜜蜂相比,俄罗斯蜜蜂具有收缩的转录反应和延迟行为成熟的转录组特征,这表明两种遗传背景之间存在不同的营养同化策略。无论饮食如何,俄罗斯蜜蜂的主要病毒病原体(DWV)丰度明显较低。最后,在微藻喂养的蜜蜂中检测到更高的体液和细胞免疫基因表达,尽管螺旋藻的免疫原性明显高于小球藻。结论遗传背景在蜜蜂对营养的反应中起关键作用。通过鉴定新的营养状态生物标志物,我们的研究结果支持了两种微藻作为功能性饲料添加剂的独特优势。此外,这些结果揭示了遗传因素在优化农业传粉媒介补充饲料时的重要性。这些发现有助于开发量身定制的营养策略和人工饲料,以改善蜜蜂的健康。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Advanced Research
Journal of Advanced Research Multidisciplinary-Multidisciplinary
CiteScore
21.60
自引率
0.90%
发文量
280
审稿时长
12 weeks
期刊介绍: Journal of Advanced Research (J. Adv. Res.) is an applied/natural sciences, peer-reviewed journal that focuses on interdisciplinary research. The journal aims to contribute to applied research and knowledge worldwide through the publication of original and high-quality research articles in the fields of Medicine, Pharmaceutical Sciences, Dentistry, Physical Therapy, Veterinary Medicine, and Basic and Biological Sciences. The following abstracting and indexing services cover the Journal of Advanced Research: PubMed/Medline, Essential Science Indicators, Web of Science, Scopus, PubMed Central, PubMed, Science Citation Index Expanded, Directory of Open Access Journals (DOAJ), and INSPEC.
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