Noninvasive Optoacoustic Imaging of Oxygen Saturation Reveals Hypoxic Hematopoietic Bone Marrow during Systemic Inflammation.

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-10-01 DOI:10.1021/acs.nanolett.5c01802

Ashish Tiwari,Narmeen Haj,Ruth Pikovsky,Shirly Hagay,Maria Berihu,Betsalel Elgrably,Liron McLey,Majd Machour,Shiri Karni-Ashkenazi,Inbar Brosh,Shy Shoham,Shulamit Levenberg,Daniel Razansky,Amir Rosenthal,Katrien Vandoorne

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Abstract

Inflammation drives various diseases, including cardiovascular, neurodegenerative, and oncological disorders, by altering immune cell dynamics in hematopoietic niches. The bone marrow is the primary site for hematopoietic stem and progenitor cell activity. Here, we present a novel, noninvasive approach using multispectral optoacoustic tomography (MSOT) to track oxygenation dynamics in the murine calvarial bone marrow during acute systemic inflammation induced by lipopolysaccharide (LPS). Our MSOT system provided real-time, label-free imaging of hemoglobin oxygen saturation (sO2), revealing significant reductions in sO2 levels in lipopolysaccharide-treated mice, indicative of increased oxygen consumption. Co-registration with microCT enabled precise vascular mapping. Hypoxia was confirmed by ex vivo Pimonidazole staining and optical imaging and was associated with elevated neutrophil counts and enhanced hematopoietic activation. These findings demonstrate MSOT's potential for noninvasive imaging of marrow oxygenation, offering insights into inflammation-driven hematopoietic activation and supporting the development of therapies targeting oxygen-sensitive pathways.

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无创光声成像显示缺氧造血骨髓全身性炎症。

炎症通过改变造血生态位中的免疫细胞动力学来驱动各种疾病，包括心血管疾病、神经退行性疾病和肿瘤疾病。骨髓是造血干细胞和祖细胞活性的主要部位。在这里，我们提出了一种新的，无创的方法，使用多光谱光声断层扫描（MSOT）来跟踪小鼠颅骨骨髓在脂多糖（LPS）诱导的急性全身炎症期间的氧合动力学。我们的MSOT系统提供了实时、无标记的血红蛋白氧饱和度（sO2）成像，显示脂多糖处理小鼠的sO2水平显著降低，表明氧气消耗增加。与微ct的联合配准实现了精确的血管制图。体外吡咪唑染色和光学成像证实缺氧与中性粒细胞计数升高和造血活化增强有关。这些发现证明了MSOT在骨髓氧合无创成像方面的潜力，为炎症驱动的造血激活提供了见解，并支持了针对氧敏感途径的治疗方法的发展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.