Magnetic Monitoring of Single-DNA Interaction Dynamics at Interfaces Using Diamond Quantum Sensors.

IF 9.1 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Nano Letters Pub Date : 2025-10-01 DOI:10.1021/acs.nanolett.5c03522

Ziting Sun,Sanyou Chen,Wanhe Li,Ruowei Ma,Fazhan Shi,Jiangfeng Du

{"title":"Magnetic Monitoring of Single-DNA Interaction Dynamics at Interfaces Using Diamond Quantum Sensors.","authors":"Ziting Sun,Sanyou Chen,Wanhe Li,Ruowei Ma,Fazhan Shi,Jiangfeng Du","doi":"10.1021/acs.nanolett.5c03522","DOIUrl":null,"url":null,"abstract":"Elucidating mechanisms of biomolecular interactions at interfaces is critical for understanding numerous physiological processes and optimizing various biomedical analysis technologies. However, inadequate sensitivity, stability, and specificity of existing detection techniques hinder interfacial biomolecular interaction studies, especially at the single-molecule level. Here, we introduce dynamic single-particle magnetic imaging (dSiPMI) leveraging nitrogen-vacancy centers in diamonds, enabling real-time magnetic detection of the dynamics of biomolecular interactions under physiological conditions. The dSiPMI visualized DNA hybridization occurring between functionalized surfaces of magnetic nanoparticle (MNP) and diamond, extracting characteristic kinetic parameters of single- and multiple-DNA interactions. Furthermore, capitalizing on the stable magnetic signature of MNPs and method robustness, we sustained monitoring of single-DNA hybridization events for 1 h through sequential measurements, revealing enhanced binding affinity correlated with increasing Na+ concentration (from 0 to 0.7 M). This study establishes a robust single-particle and single-molecule magnetic method for quantitatively analyzing the biomolecular interaction dynamics on chips and particles.","PeriodicalId":53,"journal":{"name":"Nano Letters","volume":"93 1","pages":""},"PeriodicalIF":9.1000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nano Letters","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1021/acs.nanolett.5c03522","RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Elucidating mechanisms of biomolecular interactions at interfaces is critical for understanding numerous physiological processes and optimizing various biomedical analysis technologies. However, inadequate sensitivity, stability, and specificity of existing detection techniques hinder interfacial biomolecular interaction studies, especially at the single-molecule level. Here, we introduce dynamic single-particle magnetic imaging (dSiPMI) leveraging nitrogen-vacancy centers in diamonds, enabling real-time magnetic detection of the dynamics of biomolecular interactions under physiological conditions. The dSiPMI visualized DNA hybridization occurring between functionalized surfaces of magnetic nanoparticle (MNP) and diamond, extracting characteristic kinetic parameters of single- and multiple-DNA interactions. Furthermore, capitalizing on the stable magnetic signature of MNPs and method robustness, we sustained monitoring of single-DNA hybridization events for 1 h through sequential measurements, revealing enhanced binding affinity correlated with increasing Na+ concentration (from 0 to 0.7 M). This study establishes a robust single-particle and single-molecule magnetic method for quantitatively analyzing the biomolecular interaction dynamics on chips and particles.

查看原文本刊更多论文

利用金刚石量子传感器对单dna界面相互作用动力学进行磁监测。

阐明生物分子在界面上相互作用的机制对于理解许多生理过程和优化各种生物医学分析技术至关重要。然而，现有检测技术的灵敏度、稳定性和特异性不足阻碍了界面生物分子相互作用的研究，特别是在单分子水平上。在这里，我们引入了利用金刚石中的氮空位中心的动态单粒子磁成像（dSiPMI），实现了生理条件下生物分子相互作用动态的实时磁检测。dSiPMI可视化了磁性纳米颗粒（MNP）与金刚石的功能化表面之间发生的DNA杂交，提取了单个和多个DNA相互作用的特征动力学参数。此外，利用MNPs稳定的磁性特征和方法的鲁棒性，我们通过连续测量持续监测单dna杂交事件1小时，发现随着Na+浓度的增加（从0到0.7 M），结合亲和力增强。本研究建立了一种鲁棒的单粒子和单分子磁性方法，用于定量分析芯片和粒子上的生物分子相互作用动力学。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Nano Letters 工程技术-材料科学：综合

CiteScore

16.80

自引率

2.80%

发文量

1182

审稿时长

1.4 months

期刊介绍： Nano Letters serves as a dynamic platform for promptly disseminating original results in fundamental, applied, and emerging research across all facets of nanoscience and nanotechnology. A pivotal criterion for inclusion within Nano Letters is the convergence of at least two different areas or disciplines, ensuring a rich interdisciplinary scope. The journal is dedicated to fostering exploration in diverse areas, including: - Experimental and theoretical findings on physical, chemical, and biological phenomena at the nanoscale - Synthesis, characterization, and processing of organic, inorganic, polymer, and hybrid nanomaterials through physical, chemical, and biological methodologies - Modeling and simulation of synthetic, assembly, and interaction processes - Realization of integrated nanostructures and nano-engineered devices exhibiting advanced performance - Applications of nanoscale materials in living and environmental systems Nano Letters is committed to advancing and showcasing groundbreaking research that intersects various domains, fostering innovation and collaboration in the ever-evolving field of nanoscience and nanotechnology.