Epidemiology and genetic diversity of norovirus GII genogroups among pediatric patients in Beijing, China, during 2023-2024.

IF 4 3区医学 Q2 VIROLOGY

Virology Journal Pub Date : 2025-09-29 DOI:10.1186/s12985-025-02927-z

Junhong Ai, Qiliang Li, Ke Xu, Yuxuan Li, Ying Liu, Luci Huang, Wenqi Song, Zhengde Xie

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引用次数: 0

Abstract

Background: Norovirus is an important cause of viral acute gastroenteritis (AGE) worldwide.

Methods: In order to characterize the molecular epidemiology and genetic diversity of norovirus in children in Beijing, 3634 anal swab samples of AGE patients from January 2023 to December 2024 were analyzed. Norovirus was detected using RT-PCR and genotyped by sequencing the partial RdRp and VP1 region.

Results: During the two-year period, norovirus was detected in 19.6% of AGE cases, with the highest detection rate in children under 3 years of age. GII.4 and GII.P16 were the dominant genotypes of VP1 and RdRp, with a detection rate of 36.39% and 44.59%, respectively. According to the dual-typing system combined the RdRp and VP1, the dominant genotypes of norovirus changed between 2023 and 2024. In 2023, the most common genotype was GII.3[P12] (39.15%), followed by GII.4 Sydney[P16] (32.34%) and GII.4 Sydney[P31] (15.32%). However, in 2024, the dominant genotype was GII.17[P17] (41.43%), followed by GII.4 Sydney[P16] (34.29%) and GII.3[P12] (20.0%). The GII.17 variants in this study were divided into two clusters: cluster IIIa and IIIb, which shared high nucleotide identity with GII.17 variant emerged in 2014/2015. Significantly, GII.4 Sydney[P31] and novel GII.4 Sydney[P16] variants co-circulating in this region from 2023 to 2024.

Conclusion: The data provided useful information on the molecular epidemiology of norovirus in sporadic AGE among children and highlighted the necessary to continuously monitor the epidemiological characteristics of norovirus associated AGE.

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2023-2024年北京市儿科患者诺如病毒GII基因群流行病学及遗传多样性

背景：诺如病毒是世界范围内病毒性急性胃肠炎（AGE）的重要病因。方法：对2023年1月至2024年12月北京地区儿童诺瓦克病毒的分子流行病学和遗传多样性进行分析。采用RT-PCR检测诺如病毒，并对部分RdRp区和VP1区进行测序分型。结果：2年期间，诺瓦克病毒检出率为19.6%，以3岁以下儿童检出率最高。GII.4和GII。VP1和RdRp的优势基因型为P16，检出率分别为36.39%和44.59%。根据RdRp和VP1组合的双分型系统，诺如病毒的优势基因型在2023 - 2024年间发生了变化。2023年最常见的基因型为GII.3[P12](39.15%)，其次为GII.4 Sydney[P16]（32.34%）和GII.4 Sydney[P31]（15.32%）。但在2024年，优势基因型为GII.17[P17](41.43%)，其次为GII.4 Sydney[P16]（34.29%）和GII.3[P12]（20.0%）。本研究的GII.17变异分为IIIa和IIIb两类，与2014/2015年出现的GII.17变异具有高核苷酸同源性。值得注意的是，从2023年到2024年，GII.4 Sydney[P31]和新的GII.4 Sydney[P16]变体在该地区共同流行。结论：本研究为散发性AGE儿童诺如病毒的分子流行病学研究提供了有用的信息，并强调了持续监测诺如病毒相关AGE流行病学特征的必要性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virology Journal 医学-病毒学

CiteScore

7.40

自引率

2.10%

发文量

186

审稿时长

1 months

期刊介绍： Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.