AhNHL24 enhances peanut resistance to bacterial wilt and stem rot via glutathione and phenylpropanoid pathways

IF 4.1 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Plant Science Pub Date : 2025-09-27 DOI:10.1016/j.plantsci.2025.112791

Mengtian Hou , Hao Li , Zenghui Cao , Fang Wang , Sasa Hu , Yanzhe Li , Qian Ma , Yaoyao Li , Yi Fan , Kai Zhao , Kunkun Zhao , Ding Qiu , Fangping Gong , Zhongfeng Li , Xingli Ma , Rui Ren , Dongmei Yin

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引用次数: 0

Abstract

Peanut is one of the most important food and oil crops in the world. However, the production of peanut is seriously affected by various pathogens, especially the bacterial wilt (BW) and fungal stem rot (SR) caused by Ralstonia solanacearum and Sclerotium rolfsii, respectively. Nonrace-specific disease resistance gene 1/Harpin-induced gene 1 (NDR1/HIN1)-like (NHL) family genes play crucial roles in plant defense response. Herein, a genome-wide identification of peanut AhNHL genes was conducted. Totally, 45 AhNHL genes were identified, and they were phylogenetically classified into two groups. The four genes (AhNHL14, AhNHL24, AhNHL31 and AhNHL33), exhibiting significant differences in responses to R. solanacearum infection and hormone treatments, were selected for functional characterization. Subcellular localization analysis showed that these protein fusions are primarily located on plasma membrane and/or nucleus. Meanwhile, they were transiently overexpressed in tobacco and peanut leaves, which resulted in increased resistance to R. solanacearum. Transgenic tobacco lines overexpressing AhNHL24 exhibit resistance to R. solanacearum and S. rolfsii. Further expression analysis revealed that AhNHL24 enhances the resistance to BW and SR mainly through regulating glutathione metabolism and phenylpropanoids biosynthesis. Our findings provide novel insights into roles of NHL genes in plant resistance, which would promote breeding of broad-resistant crop cultivars.

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AhNHL24通过谷胱甘肽和苯丙素途径增强花生对细菌性枯萎病和茎腐病的抗性。

花生是世界上最重要的粮食和油料作物之一。然而，花生的生产受到多种病原菌的严重影响，尤其是青枯病（BW）和真菌茎腐病（SR），分别由Ralstonia solanacearum和Sclerotium rolfsii引起。非种族特异性抗病基因1/ harpin诱导基因1 （NDR1/HIN1）样（NHL）家族基因在植物防御反应中起着重要作用。本文对花生AhNHL基因进行了全基因组鉴定。共鉴定出45个AhNHL基因，并将其系统发育分为两类。选择AhNHL14、AhNHL24、AhNHL31和AhNHL33这4个对茄青霉感染和激素处理反应有显著差异的基因进行功能表征。亚细胞定位分析表明，这些蛋白融合物主要位于质膜和/或细胞核上。同时，它们在烟草和花生叶片中短暂过表达，增强了对茄枯病菌的抗性。过表达AhNHL24的转基因烟草株系对茄枯病菌和罗氏病菌表现出抗性。进一步的表达分析表明，AhNHL24主要通过调节谷胱甘肽代谢和苯丙素的生物合成来增强对BW和SR的抗性。我们的发现为NHL基因在植物抗性中的作用提供了新的见解，这将促进作物抗宽品种的选育。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Science 生物-生化与分子生物学

CiteScore

9.10

自引率

1.90%

发文量

322

审稿时长

33 days

期刊介绍： Plant Science will publish in the minimum of time, research manuscripts as well as commissioned reviews and commentaries recommended by its referees in all areas of experimental plant biology with emphasis in the broad areas of genomics, proteomics, biochemistry (including enzymology), physiology, cell biology, development, genetics, functional plant breeding, systems biology and the interaction of plants with the environment. Manuscripts for full consideration should be written concisely and essentially as a final report. The main criterion for publication is that the manuscript must contain original and significant insights that lead to a better understanding of fundamental plant biology. Papers centering on plant cell culture should be of interest to a wide audience and methods employed result in a substantial improvement over existing established techniques and approaches. Methods papers are welcome only when the technique(s) described is novel or provides a major advancement of established protocols.