The isoprenoid biosynthesis enzyme HDS participates in chloroplast RNA editing.

IF 5.7 2区 生物学 Q1 PLANT SCIENCES
Wenjian Song, Nadia Ahmed Ali, Jia Ni, Yujia Sun, Yayi Zhang, Jiani Xing, Kexing Su, Xingxing Sun, Yizhou Jiang, Xiaobo Zhao
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Abstract

Post-transcriptional RNA editing and retrograde signaling in chloroplasts are crucial for coordinating gene expression between chloroplasts and the nucleus in flowering plants, yet the molecular link between them remains poorly understood. This study reveals that the Arabidopsis isoprenoid biosynthesis enzyme, 1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase (HDS), a known regulator of retrograde signaling, is also involved in chloroplast RNA editing. We found that the loss of HDS function significantly altered RNA editing efficiency at multiple specific sites in chloroplast transcripts. HDS mutants exhibited a pale phenotype and early seedling lethality, with severely impaired chloroplast development and photosynthetic apparatus assembly. Moreover, we demonstrated that HDS physically interacts with chloroplast multiple-site RNA editing factors MORF2 and MORF9 and participates in RNA editing by modulating their dimerization. Taken together, our work indicates that the important retrograde signaling regulator HDS also plays a novel role in chloroplast RNA editing, providing insight into the connection between organelle-to-nucleus communication and RNA metabolism in chloroplasts.

类异戊二烯生物合成酶HDS参与叶绿体RNA编辑。
在开花植物中,叶绿体中的转录后RNA编辑和逆行信号对于协调叶绿体和细胞核之间的基因表达至关重要,但它们之间的分子联系尚不清楚。这项研究表明,拟南芥类异戊二烯生物合成酶1-羟基-2-甲基-2-(E)-丁烯基-4-二磷酸合成酶(HDS)是一种已知的逆行信号调节因子,也参与叶绿体RNA编辑。我们发现HDS功能的缺失显著改变了叶绿体转录本中多个特定位点的RNA编辑效率。HDS突变体表现出苍白的表型和早期幼苗致死率,叶绿体发育和光合机构组装严重受损。此外,我们证明HDS与叶绿体多位点RNA编辑因子MORF2和MORF9物理相互作用,并通过调节它们的二聚化参与RNA编辑。综上所述,我们的研究表明,重要的逆行信号调节因子HDS在叶绿体RNA编辑中也起着新的作用,为叶绿体中细胞器与细胞核之间的通信和RNA代谢之间的联系提供了新的见解。
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来源期刊
Journal of Experimental Botany
Journal of Experimental Botany 生物-植物科学
CiteScore
12.30
自引率
4.30%
发文量
450
审稿时长
1.9 months
期刊介绍: The Journal of Experimental Botany publishes high-quality primary research and review papers in the plant sciences. These papers cover a range of disciplines from molecular and cellular physiology and biochemistry through whole plant physiology to community physiology. Full-length primary papers should contribute to our understanding of how plants develop and function, and should provide new insights into biological processes. The journal will not publish purely descriptive papers or papers that report a well-known process in a species in which the process has not been identified previously. Articles should be concise and generally limited to 10 printed pages.
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