A clever evaluation of the antidiabetic medications linagliptin and empagliflozin in bulk and spiked urine samples.

IF 1.8 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Bioanalysis Pub Date : 2025-09-29 DOI:10.1080/17576180.2025.2567231

Osama I Abdel Sattar, Hamed H M Abuseada, Mohamed S Emara, Islam Selim, Nahla A Abdelshafi

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引用次数: 0

Abstract

Background: The objective of this research is to provide differential pulse voltammetry (DPV) method that is an ingenious, simple, sensitive, and selective method for the quantitative analysis of linagliptin and empagliflozin in spiked human urine samples. Faster analysis times and the use of small sample volumes are great advantages of DPV.

Research design and methods: Before centrifuging the samples for 5 min at 3000 rpm, methanol was added to precipitate and remove any suspended compounds. As a working electrode, glassy carbon electrode (GCE) was employed. The auxiliary electrode was a platinum electrode, while the reference electrode was Ag|AgCl|KCl(sat.). To maximize the experimental conditions for simultaneous determination, the relationship between the current, pH and scan rate was examined.

Results: Optimal conditions for quantitative determination were obtained in a phosphate buffer (PB) at pH 7. Plotting LIN and EMP concentrations against the DPV peak height revealed good linearity. It was found that the linear ranges for LIN and EMP were 10-90 and 10-70 µM, respectively.

Conclusions: This approach has been designed for effectively estimating the levels of examined drugs in human urine without matrix effect. The obtained results showed a good recovery values of both drugs.

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一个聪明的评估抗糖尿病药物利格列汀和恩格列净在散装和加标尿样。

背景：本研究的目的是建立一种新颖、简便、灵敏、选择性好的差分脉冲伏安法（DPV），用于人尿液中利格列汀和恩格列净的定量分析。更快的分析时间和使用小样本量是DPV的巨大优势。研究设计与方法：样品在3000rpm下离心5min前，加入甲醇沉淀去除悬浮物。工作电极采用玻碳电极（GCE）。辅助电极为铂电极，参比电极为Ag|AgCl|KCl（sat.）。为了优化同时测定的实验条件，考察了电流、pH和扫描速率之间的关系。结果：在pH为7的磷酸盐缓冲液（PB）中获得了最佳的定量条件。LIN和EMP浓度与DPV峰高的关系显示出良好的线性关系。结果表明，LIN和EMP的线性范围分别为10 ~ 90µM和10 ~ 70µM。结论：该方法可有效地估计人体尿液中被检药物的水平，无基质效应。结果表明，两种药物均具有良好的回收率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioanalysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

3.30

自引率

16.70%

发文量

审稿时长

2 months

期刊介绍： Reliable data obtained from selective, sensitive and reproducible analysis of xenobiotics and biotics in biological samples is a fundamental and crucial part of every successful drug development program. The same principles can also apply to many other areas of research such as forensic science, toxicology and sports doping testing. The bioanalytical field incorporates sophisticated techniques linking sample preparation and advanced separations with MS and NMR detection systems, automation and robotics. Standards set by regulatory bodies regarding method development and validation increasingly define the boundaries between speed and quality. Bioanalysis is a progressive discipline for which the future holds many exciting opportunities to further reduce sample volumes, analysis cost and environmental impact, as well as to improve sensitivity, specificity, accuracy, efficiency, assay throughput, data quality, data handling and processing. The journal Bioanalysis focuses on the techniques and methods used for the detection or quantitative study of analytes in human or animal biological samples. Bioanalysis encourages the submission of articles describing forward-looking applications, including biosensors, microfluidics, miniaturized analytical devices, and new hyphenated and multi-dimensional techniques. Bioanalysis delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for the modern bioanalyst.