Bacteriophages combined with hybridized nanoflower-based electrochemical biosensor for the specific and rapid detection of live Acinetobacter baumannii in real samples

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta Pub Date : 2025-09-30 DOI:10.1007/s00604-025-07562-7

Huan Wang, Wajid Hussain, Xiaohan Yang, Yanming Chen, Tong Hu, Wei Chen, Shenqi Wang

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Abstract

Acinetobacter baumannii is an opportunistic nosocomial bacterial pathogen that causes significant global morbidity and mortality. A rapid and accurate detection platform is essential to minimize the risk of nosocomial infections and drug resistance. We developed an innovative electrochemical biosensor for the rapid detection of A. baumannii in real samples, using a unique enzyme immobilization method. The biosensor is capable of identifying A. baumannii through the specific phages attached to HRP@Cu₃(PO₄)₂ hybrid nanoflower with the help of AuNPs, creating a bifunctional complex that offers both biomolecular recognition and signal amplification. The developed phage-based electrochemical biosensor was efficient in detecting live A. baumannii across a range of 5.77 × 10²–5.77 × 10⁷ CFU/mL, up to 3 CFU/mL, within 30 min in serum and plasma samples. The biosensor employs a straightforward design, offering more simple operation and higher specificity than traditional methods, such as culturing. Furthermore, the immobilization of HRP on the hybrid nanoflowers improved its stability against environmental conditions and affinity for the substrate. The findings of this study demonstrate that electrochemical biosensors based on HRP-hybrid nanoflowers and phages can be effectively utilized for the rapid detection of A. baumannii while also laying the groundwork for stable HRP immobilization.

Graphical Abstract

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噬菌体结合杂交纳米花电化学生物传感器特异性快速检测真实样品中的活鲍曼不动杆菌。

鲍曼不动杆菌是一种机会性医院细菌性病原体，在全球范围内引起显著的发病率和死亡率。快速准确的检测平台对于最大限度地减少医院感染和耐药风险至关重要。我们开发了一种创新的电化学生物传感器，用于快速检测真实样品中的鲍曼不动杆菌，采用独特的酶固定化方法。该生物传感器能够在AuNPs的帮助下，通过附着在HRP@Cu3(PO4)2杂交纳米花上的特定噬菌体识别鲍曼不动杆菌，从而形成具有生物分子识别和信号放大功能的双功能复合物。基于噬菌体的电化学生物传感器可在30 min内检测出血清和血浆中5.77 × 102 ~ 5.77 × 107 CFU/mL，最大3 CFU/mL的活鲍曼不动杆菌。这种生物传感器采用直接的设计，比传统的方法（如培养）操作更简单，特异性更高。此外，HRP固定在杂交纳米花上提高了其对环境条件的稳定性和对底物的亲和力。本研究结果表明，基于HRP杂交纳米花和噬菌体的电化学生物传感器可以有效地用于鲍曼不动杆菌的快速检测，同时也为HRP的稳定固定奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Microchimica Acta 化学-分析化学

CiteScore

9.80

自引率

5.30%

发文量

410

审稿时长

2.7 months

期刊介绍： As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.