Development of a Cell Membrane Conformational Cannabinoid CB₁ Receptor Biosensor for the Detection of Synthetic Cannabinoids.

IF 9.1 1区化学 Q1 CHEMISTRY, ANALYTICAL

ACS Sensors Pub Date : 2025-09-29 DOI:10.1021/acssensors.4c03589

Michelle Glass, Nahid Khalajiassadi, Shuli Chen, Dharshini Ganeshan, Hannah Partington, David B Finlay

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引用次数: 0

Abstract

This study aimed to develop a chemical structure-independent, sensitive screening platform for detecting novel synthetic cannabinoids using isolated cell membranes. Resonance energy transfer techniques were employed to detect conformational changes in the CB₁ receptor upon agonist binding. Eight cannabinoid CB₁ conformational biosensors were generated and characterized featuring different tag compositions and placements. The biosensors were initially tested in live cell assays, before conditions were optimized for activity in isolated cell membranes. The optimal sensor developed could successfully distinguish between different agonist efficacy and potency profiles. This biosensor allows for the detection of synthetic cannabinoids without relying on their chemical structure, potentially providing a valuable tool for compliance agencies to rapidly respond to the proliferation of new psychoactive substances. The ability to differentiate efficacy and potency profiles may also contribute to harm reduction strategies upon detection of these compounds.

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用于合成大麻素检测的细胞膜构象大麻素CB1受体生物传感器的研制

本研究旨在建立一种化学结构无关的、灵敏的筛选平台，用于分离细胞膜检测新型合成大麻素。共振能量转移技术用于检测CB1受体在激动剂结合后的构象变化。合成了8个大麻素CB1构象生物传感器，并对其进行了表征，这些传感器具有不同的标签组成和放置位置。生物传感器最初在活细胞试验中进行测试，然后在分离细胞膜中优化条件以获得活性。所开发的最优传感器可以成功区分不同的激动剂的功效和效力谱。这种生物传感器允许在不依赖其化学结构的情况下检测合成大麻素，可能为合规机构提供有价值的工具，以快速响应新的精神活性物质的扩散。区分功效和效力谱的能力也可能有助于在检测这些化合物时采取减少危害的策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Sensors Chemical Engineering-Bioengineering

CiteScore

14.50

自引率

3.40%

发文量

372

期刊介绍： ACS Sensors is a peer-reviewed research journal that focuses on the dissemination of new and original knowledge in the field of sensor science, particularly those that selectively sense chemical or biological species or processes. The journal covers a broad range of topics, including but not limited to biosensors, chemical sensors, gas sensors, intracellular sensors, single molecule sensors, cell chips, and microfluidic devices. It aims to publish articles that address conceptual advances in sensing technology applicable to various types of analytes or application papers that report on the use of existing sensing concepts in new ways or for new analytes.