Exogenous nitric oxide enhances waterlogging tolerance in Boehmeria nivea L. through modulating BnCAT3 expression

IF 6.2 1区农林科学 Q1 AGRICULTURAL ENGINEERING

Industrial Crops and Products Pub Date : 2025-09-30 DOI:10.1016/j.indcrop.2025.121967

Shiwei Yan, Yu Chen, Aiguo Zhu, Ping Chen, Xiaofei Wang, Kunmei Chen, Jia Chen, Gang Gao, Haohan Zhao, Peng Chen, Jikang Chen

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Abstract

Waterlogging was one of the primarily factors affecting growth and yield of crops. As a gaseous signaling molecule, nitric oxide (NO) pays important roles in stress response and has been successfully applied in various plants to improve the tolerance under waterlogging, while there was little research in ramie. To investigate the function of exogenous NO application in ramie under waterlogging, pot experiments were carried out using ramie cultivar Zhongzhu NO.2 in this study. Three treatments, waterlogging stress (W), waterlogging stress with exogenous sodium nitroprusside (SNP) application (W+SNP), and control group with normal irrigation (CK), were established with both 1-year-old ramie (1 R) and 2-year-old ramie (2 R) plants. The results showed that NO application alleviated waterlogging stress in ramie significantly by decreasing the losses in plant height, leaf area, relative water content in leaf, SPAD value and net photosynthetic rate by 2.31 ∼ 3.17 %, 4.99 ∼ 5.77 %, 1.69 ∼ 2.93 %, 4.48 ∼ 9.93 % and 7.15 ∼ 9.33 %, respectively. Accumulation of NO under waterlogging with SNP spraying was observed by 1.7 ∼ 2.9 times, enhancing antioxidant enzyme activities by 6.75∼37.93 % and modulating osmoregulatory substances (decrease in soluble sugar and malondialdehyde but increase in proline), which resulted in the better performance of plant growth. Catalase (CAT) activity was highly positively correlated with NO, while molecular analysis identified three members of BnCATs in ramie which was up-regulated obviously with exogenous NO application under waterlogging. BnCAT3 was detected to be a candidate gene for further study, which was significantly regulated by NO content, highly expressed in all the tissues and responding positively to waterlogging, NaCl, PEG and ABA stresses. These results provided new insight for genetic breeding and application of resistance in ramie under waterlogging.

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外源一氧化氮通过调节BnCAT3的表达增强了Boehmeria nivea L.的耐涝能力

内涝是影响作物生长和产量的主要因素之一。一氧化氮（NO）作为一种气体信号分子，在胁迫响应中起着重要的作用，已成功应用于多种植物，以提高植物对涝害的耐受性，但对苎麻的研究较少。为研究外源NO在涝渍条件下对苎麻的作用，以苎麻品种中竹2号为试验材料，进行盆栽试验。以1年生苎麻（1 R）和2年生苎麻（2 R）植株为试验材料，设置涝渍胁迫(W)、外源硝普钠（SNP）胁迫（W+SNP）和正常灌溉（CK） 3个处理。结果表明，施用NO可显著缓解苎麻涝渍胁迫，使其株高、叶面积、叶片相对含水量、SPAD值和净光合速率分别降低2.31 ~ 3.17 %、4.99 ~ 5.77 %、1.69 ~ 2.93 %、4.48 ~ 9.93 %和7.15 ~ 9.33 %。单核苷酸多态性（SNP）涝渍处理下NO积累次数为1.7 ~ 2.9次，抗氧化酶活性提高6.75 ~ 37.93 %，渗透调节物质（可溶性糖和丙二醛降低，脯氨酸增加）得到调节，提高植株生长性能。过氧化氢酶（CAT）活性与NO呈高度正相关，分子分析发现，内涝条件下，苎麻过氧化氢酶（CAT）有3个成员在外源NO的作用下明显上调。BnCAT3基因受NO含量的显著调控，在各组织中均有高表达，对涝渍、NaCl、PEG和ABA胁迫均有积极响应，是有待进一步研究的候选基因。这些结果为苎麻抗涝遗传育种和抗涝应用提供了新的思路。

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来源期刊

Industrial Crops and Products 农林科学-农业工程

CiteScore

9.50

自引率

8.50%

发文量

1518

审稿时长

43 days

期刊介绍： Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.